| Estimation of Coxiella burnetii prevalence in dairy cattle in intensive systems by serological and molecular analyses of bulk-tank milk samples. | |
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MedLine Citation:
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PMID: 22459811 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A large-scale investigation on Coxiella burnetii was carried out in dairy cattle herds from a Q fever-endemic region to evaluate the degree of exposure to C. burnetii and to estimate prevalences. This study included all of the dairy cattle herds from the province of Bizkaia, Northern Spain (n=178). Herds were visited between September 2009 and February 2010, and 100mL of bulk-tank milk (BTM) per farm was collected to be analyzed by ELISA and PCR. Blood samples were also taken from about 15 animals randomly selected from each herd. One hundred nineteen of the 178 studied herds (66.9±6.9%) were positive for the presence of anti-C. burnetii antibodies in BTM. Serum samples from 1,306 cows, 654 heifers, and 502 calves were analyzed by ELISA, and cows showed a statistically significantly higher seroprevalence (12.3±1.8%) than heifers (1.1±0.8%) and calves (0.0±0.0%). Eighty-nine herds (50.0±7.3%) had at least 1 seropositive animal, but within-herd prevalences higher than 20% were only observed in 24 herds (13.5±5.0%). A significant correlation was observed between BTM ELISA sample-to-positive control ratios and within-herd seroprevalence, being higher when considering only cows (R(2)=0.21). Animals from herds with negative BTM by ELISA showed a mean seroprevalence of 2.5%, whereas animals from herds with positive BTM samples had a statistically significantly higher seroprevalence (8.9%, F=19.7, degrees of freedom=1). The proportion of herds C. burnetii positive by BTM PCR was 51.7±7.3% (92/178). The widespread distribution of C. burnetii in cattle advocates for the implementation of Q fever control strategies. |
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Authors:
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I Astobiza; F Ruiz-Fons; A Piñero; J F Barandika; A Hurtado; A L García-Pérez |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of dairy science Volume: 95 ISSN: 1525-3198 ISO Abbreviation: J. Dairy Sci. Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-03-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2985126R Medline TA: J Dairy Sci Country: United States |
Other Details:
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Languages: eng Pagination: 1632-8 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 American Dairy Science Association. Published by Elsevier Inc. All rights reserved. |
Affiliation:
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NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario, Department of Animal Health, Berreaga 1, 48160 Derio, Bizkaia, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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