Document Detail


Estimating glucose metabolism using glucose analogs and two tracer kinetic models in isolated rabbit heart.
MedLine Citation:
PMID:  9683457     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this investigation was to 1) evaluate the relative accuracy of the Sokoloff and Patlak tracer kinetic models in estimating glucose metabolic rate (GMR) in the presence and absence of insulin; 2) evaluate the effect of nutritional state on the lumped constant (LC); and 3) compare the kinetics of 2-fluoro-2-deoxy-D-[14C]glucose (FDG) and 2-deoxy-D-[3H]glucose (DG) membrane transport and phosphorylation. The experimental preparation was the isolated, red blood cell-albumin-perfused rabbit heart. Our results showed that both tracer kinetic models provided GMR estimates that correlated well with the Fick method (for FDG, R = 0. 84 and 0.91 for the Sokoloff and Patlak models, respectively); nutritional state did not affect the LC; and FDG and DG have different transport and/or phosphorylation parameters. We also observed that 1) the addition of a fourth compartment to the Sokoloff model reduced the mean squared error between measured and modeled data by a factor of 7.4; 2) a longer time (21.8 min) was required to obtain a linear phase of the Patlak plot than is allowed in clinical studies; and 3) accurate GMR estimates were obtained only by using different LCs reflecting insulin's presence or absence. Our results indicate potential sources of error in the use of FDG and positron emission tomography to quantify GMR in patients.
Authors:
R C Marshall; P Powers-Risius; R H Huesman; B W Reutter; S E Taylor; H E Maurer; M K Huesman; T F Budinger
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  275     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1998 Aug 
Date Detail:
Created Date:  1998-09-16     Completed Date:  1998-09-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H668-79     Citation Subset:  IM    
Affiliation:
Lawrence Berkeley National Laboratory, University of California, Center for Functional Imaging, Berkeley, CA 94720, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Carbon Radioisotopes
Deoxyglucose / pharmacokinetics*
Eating
Erythrocytes
Fasting
Fluorodeoxyglucose F18 / pharmacokinetics*
Glucose / analogs & derivatives,  metabolism*
Heart / drug effects,  physiology
Insulin / pharmacology
Iodine Radioisotopes
Kinetics
Male
Mathematics
Models, Cardiovascular*
Myocardium / metabolism*
Perfusion
Rabbits
Radioisotope Dilution Technique
Regression Analysis
Tritium
Grant Support
ID/Acronym/Agency:
P01 HL-25840/HL/NHLBI NIH HHS; R01 HL-48068/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Iodine Radioisotopes; 10028-17-8/Tritium; 11061-68-0/Insulin; 154-17-6/Deoxyglucose; 50-99-7/Glucose; 63503-12-8/Fluorodeoxyglucose F18

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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