| Esterification of polyglycerol with polycondensed ricinoleic acid catalysed by immobilised Rhizopus oryzae lipase. | |
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MedLine Citation:
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PMID: 23263570 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The enzymatic method for synthesising polyglycerol polyricinoleate (PGPR), a food additive named E-476, was successfully carried out in the presence of immobilised Rhizopus oryzae lipase in a solvent-free medium. The great advantage of using the commercial preparation of R. oryzae lipase is that it is ten times cheaper than the commercial preparation of R. arrhizus lipase, the biocatalyst used in previous studies. The reaction, which is really a reversal of hydrolysis, takes place in the presence of a very limited amount of aqueous phase. Immobilisation of the lipase by physical adsorption onto an anion exchange resin provided good results in terms of activity, enzyme stability and reuse of the immobilised derivative. It has been established that the adsorption of R. oryzae lipase on Lewatit MonoPlus MP 64 follows a pseudo-second order kinetics, which means that immobilisation is a process of chemisorption, while the equilibrium adsorption follows a Langmuir isotherm. The use of this immobilised derivative as catalyst for obtaining PGPR under a controlled atmosphere in a vacuum reactor, with a dry nitrogen flow intake, allowed the synthesis of a product with an acid value lower than 6 mg KOH/g, which complies with the value established by the European Commission Directive. This product also fulfils the European specifications regarding the hydroxyl value and refractive index given for this food additive, one of whose benefits, as proved in our experiments, is that it causes a drastic decrease in the viscosity (by 50 %) and yield stress (by 82 %) of chocolate, comparable to the impact of customary synthesised PGPR. |
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Authors:
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S Ortega; M F Máximo; M C Montiel; M D Murcia; G Arnold; J Bastida |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-12-23 |
Journal Detail:
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Title: Bioprocess and biosystems engineering Volume: - ISSN: 1615-7605 ISO Abbreviation: Bioprocess Biosyst Eng Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088505 Medline TA: Bioprocess Biosyst Eng Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Chemical Engineering, University of Murcia, Campus de Espinardo, 30100, Murcia, Spain, dortega@um.es. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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