Document Detail

Esterification of phenolic acids catalyzed by lipases immobilized in organogels.
MedLine Citation:
PMID:  20490615     Owner:  NLM     Status:  MEDLINE    
Lipases from Rhizomucor miehei and Candida antarctica B were immobilized in hydroxypropylmethyl cellulose organogels based on surfactant-free microemulsions consisting of n-hexane, 1-propanol and water. Both lipases kept their catalytic activity, catalyzing the esterification reactions of various phenolic acids including cinnamic acid derivatives. High reaction rates and yields (up to 94%) were obtained when lipase from C. antarctica was used. Kinetic studies have been performed and apparent kinetic constants were determined showing that ester synthesis catalyzed by immobilized lipases occurs via the Michaelis-Menten mechanism.
M Zoumpanioti; E Merianou; T Karandreas; H Stamatis; A Xenakis
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Publication Detail:
Type:  Journal Article     Date:  2010-05-21
Journal Detail:
Title:  Biotechnology letters     Volume:  32     ISSN:  1573-6776     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-17     Completed Date:  2011-01-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1457-62     Citation Subset:  IM    
Institute of Biological Research and Biotechnology, National Hellenic Research Foundation, Athens, Greece.
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MeSH Terms
Candida / enzymology*
Enzymes, Immobilized / metabolism*
Hydroxybenzoic Acids / metabolism*
Lipase / isolation & purification,  metabolism*
Rhizomucor / enzymology*
Reg. No./Substance:
0/Enzymes, Immobilized; 0/Gels; 0/Hydroxybenzoic Acids; 29656-58-4/phenolic acid; EC

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