| Esterification of monohydroxyfatty acids into the lipids of a macrophage cell line. | |
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MedLine Citation:
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PMID: 6417729 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cells of a mouse macrophage-like tumor cell line, J774.2, were incubated with 0.6 microM radiolabeled mono- and di-hydroxyfatty acids. Monohydroxyfatty acid products of the neutrophil and platelet lipoxygenase pathways (5-HETE, 15-HETE, and 12-HETE) were rapidly taken up (42-64% of the counts cell associated at 1 min) and esterified into triglycerides and phospholipids. 5-HETE and 12-HETE were found in triglycerides and distributed among phospholipid classes while 50% of added 15-HETE was esterified into phosphatidyl inositol. Treatment of phospholipids from cells incubated with 5-HETE, 12-HETE, and 15-HETE with phospholipase A2 resulted in release of the respective monohydroxyfatty acid. HHT, a monohydroxyfatty acid product of the cyclooxygenase pathway, was taken up and esterified more slowly than the lipoxygenase products. In addition, HHT was not released when the phospholipids from cells incubated with HHT were treated with phospholipase A2. LTB4, a dihydroxyfatty acid product of neutrophil lipoxygenase, was not taken up by J774.2 cells. The unique patterns of uptake and intracellular distribution of the different monohydroxyfatty acids suggests that the enzymes involved in the esterification of these compounds have substrate specificity and may also relate to the specific biologic effects of these compounds. |
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Authors:
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W F Stenson; M W Nickells; J P Atkinson |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Prostaglandins Volume: 26 ISSN: 0090-6980 ISO Abbreviation: Prostaglandins Publication Date: 1983 Aug |
Date Detail:
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Created Date: 1984-01-27 Completed Date: 1984-01-27 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0320271 Medline TA: Prostaglandins Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 253-64 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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12-Hydroxy-5,8,10,14-eicosatetraenoic Acid Animals Arachidonic Acid Arachidonic Acids / metabolism Cell Line Fatty Acids / metabolism* Hydroxyeicosatetraenoic Acids* Lipid Metabolism* Macrophages / metabolism* Mice Phospholipids / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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1 K08 AM00811/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Arachidonic Acids; 0/Fatty Acids; 0/Hydroxyeicosatetraenoic Acids; 0/Phospholipids; 506-32-1/Arachidonic Acid; 59985-28-3/12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; 72255-35-7/5-hydroxy-6,8,11,14-eicosatetraenoic acid; 73945-47-8/15-hydroxy-5,8,11,13-eicosatetraenoic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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