Document Detail


Esterification of monohydroxyfatty acids into the lipids of a macrophage cell line.
MedLine Citation:
PMID:  6417729     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cells of a mouse macrophage-like tumor cell line, J774.2, were incubated with 0.6 microM radiolabeled mono- and di-hydroxyfatty acids. Monohydroxyfatty acid products of the neutrophil and platelet lipoxygenase pathways (5-HETE, 15-HETE, and 12-HETE) were rapidly taken up (42-64% of the counts cell associated at 1 min) and esterified into triglycerides and phospholipids. 5-HETE and 12-HETE were found in triglycerides and distributed among phospholipid classes while 50% of added 15-HETE was esterified into phosphatidyl inositol. Treatment of phospholipids from cells incubated with 5-HETE, 12-HETE, and 15-HETE with phospholipase A2 resulted in release of the respective monohydroxyfatty acid. HHT, a monohydroxyfatty acid product of the cyclooxygenase pathway, was taken up and esterified more slowly than the lipoxygenase products. In addition, HHT was not released when the phospholipids from cells incubated with HHT were treated with phospholipase A2. LTB4, a dihydroxyfatty acid product of neutrophil lipoxygenase, was not taken up by J774.2 cells. The unique patterns of uptake and intracellular distribution of the different monohydroxyfatty acids suggests that the enzymes involved in the esterification of these compounds have substrate specificity and may also relate to the specific biologic effects of these compounds.
Authors:
W F Stenson; M W Nickells; J P Atkinson
Related Documents :
387819 - Metabolism of arachidonic acid in ionophore-stimulated neutrophils. esterification of a...
6439809 - Inhibition of phytohemagglutinin-induced lymphocyte mitogenesis by lipoxygenase metabol...
3031819 - The role of arachidonic acid metabolites in preeclampsia.
3929789 - Immunochemical study on the contribution of hypolipidaemic-induced cytochrome p-452 to ...
2471269 - Arachidonic acid and other fatty acids directly activate potassium channels in smooth m...
6099279 - The production of arachidonate lipoxygenase products by rat intestine is increased by p...
912029 - Analysis of hexosaminitol-containing disaccharide alditols from rat brain glycoproteins...
6414459 - Measles-virus-persistent infection in bgm cells. modification of the incorporation of [...
11988079 - Isolation and characterization of a polymerized prion protein.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Prostaglandins     Volume:  26     ISSN:  0090-6980     ISO Abbreviation:  Prostaglandins     Publication Date:  1983 Aug 
Date Detail:
Created Date:  1984-01-27     Completed Date:  1984-01-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0320271     Medline TA:  Prostaglandins     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  253-64     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Animals
Arachidonic Acid
Arachidonic Acids / metabolism
Cell Line
Fatty Acids / metabolism*
Hydroxyeicosatetraenoic Acids*
Lipid Metabolism*
Macrophages / metabolism*
Mice
Phospholipids / metabolism
Grant Support
ID/Acronym/Agency:
1 K08 AM00811/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Fatty Acids; 0/Hydroxyeicosatetraenoic Acids; 0/Phospholipids; 506-32-1/Arachidonic Acid; 59985-28-3/12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; 72255-35-7/5-hydroxy-6,8,11,14-eicosatetraenoic acid; 73945-47-8/15-hydroxy-5,8,11,13-eicosatetraenoic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Effect of different prostaglandin synthesis inhibitors on post-occlusive blood flow in human forearm...
Next Document:  Dipyridamole interferes with the incorporation of arachidonic acid and stimulates prostacyclin produ...