Document Detail

Esterification of monohydroxyfatty acids into the lipids of a macrophage cell line.
MedLine Citation:
PMID:  6417729     Owner:  NLM     Status:  MEDLINE    
Cells of a mouse macrophage-like tumor cell line, J774.2, were incubated with 0.6 microM radiolabeled mono- and di-hydroxyfatty acids. Monohydroxyfatty acid products of the neutrophil and platelet lipoxygenase pathways (5-HETE, 15-HETE, and 12-HETE) were rapidly taken up (42-64% of the counts cell associated at 1 min) and esterified into triglycerides and phospholipids. 5-HETE and 12-HETE were found in triglycerides and distributed among phospholipid classes while 50% of added 15-HETE was esterified into phosphatidyl inositol. Treatment of phospholipids from cells incubated with 5-HETE, 12-HETE, and 15-HETE with phospholipase A2 resulted in release of the respective monohydroxyfatty acid. HHT, a monohydroxyfatty acid product of the cyclooxygenase pathway, was taken up and esterified more slowly than the lipoxygenase products. In addition, HHT was not released when the phospholipids from cells incubated with HHT were treated with phospholipase A2. LTB4, a dihydroxyfatty acid product of neutrophil lipoxygenase, was not taken up by J774.2 cells. The unique patterns of uptake and intracellular distribution of the different monohydroxyfatty acids suggests that the enzymes involved in the esterification of these compounds have substrate specificity and may also relate to the specific biologic effects of these compounds.
W F Stenson; M W Nickells; J P Atkinson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Prostaglandins     Volume:  26     ISSN:  0090-6980     ISO Abbreviation:  Prostaglandins     Publication Date:  1983 Aug 
Date Detail:
Created Date:  1984-01-27     Completed Date:  1984-01-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0320271     Medline TA:  Prostaglandins     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  253-64     Citation Subset:  IM    
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MeSH Terms
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Arachidonic Acid
Arachidonic Acids / metabolism
Cell Line
Fatty Acids / metabolism*
Hydroxyeicosatetraenoic Acids*
Lipid Metabolism*
Macrophages / metabolism*
Phospholipids / metabolism
Grant Support
Reg. No./Substance:
0/Arachidonic Acids; 0/Fatty Acids; 0/Hydroxyeicosatetraenoic Acids; 0/Phospholipids; 506-32-1/Arachidonic Acid; 59985-28-3/12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; 72255-35-7/5-hydroxy-6,8,11,14-eicosatetraenoic acid; 73945-47-8/15-hydroxy-5,8,11,13-eicosatetraenoic acid

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