Document Detail

Establishment of two morphologically distinct PC12 cell lines resistant to 25-OH-cholesterol toxicity.
MedLine Citation:
PMID:  9344846     Owner:  NLM     Status:  MEDLINE    
Two novel populations of spontaneous PC12 cell mutants resistant to a toxic concentration of 25-OH-cholesterol (5 microg/ml, 12.5 microM) were isolated and designated as R25R and F25R based on cell morphology. R25R consisted of round cells that were morphologically similar to the parent PC12 cells, and responded to nerve growth factor by extending neurites. F25R was a group of process-bearing flat cells that did not assume a neuronal morphology in the presence of nerve growth factor. These two cell lines also acquired some cross-resistance toward other cholesterol oxides. Nerve growth factor induced prominent voltage-dependent calcium currents in parent PC12 cells and in R25R, but not in F25R. Further experiments indicated that the parent PC12 cells, R25R and F25R exhibited different properties when challenged with a variety of toxic insults, including amphotericin B, serum withdrawal and beta-amyloid protein treatment.
J Y Chang; K D Phelan; L Z Liu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  239     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1997-11-17     Completed Date:  1997-11-17     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  429-34     Citation Subset:  IM    
Copyright Information:
Copyright 1997 Academic Press.
Department of Anatomy, University of Arkansas for Medical Sciences, Little Rock 72205, USA.
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MeSH Terms
Amyloid beta-Protein / toxicity
Calcium Channels / drug effects
Cell Culture Techniques / methods
Cell Death / drug effects
Cell Differentiation / drug effects
Cholesterol / analogs & derivatives,  pharmacology
Culture Media, Serum-Free
Drug Resistance, Neoplasm
Hydroxycholesterols / toxicity*
Ion Channel Gating / drug effects
Membrane Lipids / chemistry
Nerve Growth Factors / pharmacology
PC12 Cells / drug effects*
Grant Support
Reg. No./Substance:
0/Amyloid beta-Protein; 0/Calcium Channels; 0/Culture Media, Serum-Free; 0/Hydroxycholesterols; 0/Membrane Lipids; 0/Nerve Growth Factors; 1250-95-9/cholesterol alpha-oxide; 2140-46-7/25-hydroxycholesterol; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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