| Establishment of two chicken embryonic cell lines in a newly developed nutrient medium. | |
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MedLine Citation:
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PMID: 21213599 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two cell lines, named KCEK and KCEL, were established from chicken embryonic kidney and lung. The basal culture medium was newly developed and the cell growth medium consisted of K1999 supplemented with 10% heat inactivated chicken serum. Both cells were well adapted to grow in vitro and more than 50 passages have been made so far. Once the cell lines were established the cells were easily adapted to grow in other growth media supplemented with fetal calf serum. Neither tumor formation in chicks nor P52 avian leucosis common antigen was detected in these cells. However, the oncogene analysis on these cells has not been performed yet. Both cells were permissive hosts for the Aujeszky's disease virus, Newcastle disease virus, and vesicular stomatitis virus. |
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Authors:
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Katsuyuki Kadoi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The new microbiologica Volume: 33 ISSN: 1121-7138 ISO Abbreviation: New Microbiol. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2011-01-07 Completed Date: 2011-02-08 Revised Date: 2011-02-25 |
Medline Journal Info:
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Nlm Unique ID: 9516291 Medline TA: New Microbiol Country: Italy |
Other Details:
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Languages: eng Pagination: 393-7 Citation Subset: IM |
Affiliation:
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Laboratory of Veterinary Microbiology, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa, Japan. katsuyukikadoi@yahoo.co.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Culture Techniques Cell Line* Chick Embryo / cytology* Culture Media / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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