Document Detail


Establishment of tsA58 transgenic rats as a source of conditionally immortalized cell lines with differentiated functions.
MedLine Citation:
PMID:  12012717     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To isolate a variety of rat cell lines with differentiated functions, we developed transgenic rat lines that ubiquitously express the temperature-sensitive large T-antigen gene of the simian virus 40 (SV40) tsA58 mutant under the control of the SV40 large T-antigen promoter. These rats might be advantageous for simultaneously establishing cell lines from different tissues of rats with the same genetic origin. The transgenic rat lines transmit a functional copy of the transgene and were bred with sib mating to generate the homozygous transgene. The established cell lines from this transgenic rat had temperature dependent growth and retained some of the differentiated functions of each particular tissue, and were useful as a ready source of novel conditionally immortalized cell lines. The possible use and perspectives of these transgenic cell lines are discussed.
Authors:
Ri-ichi Takahashi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experimental animals / Japanese Association for Laboratory Animal Science     Volume:  51     ISSN:  1341-1357     ISO Abbreviation:  Exp. Anim.     Publication Date:  2002 Apr 
Date Detail:
Created Date:  2002-05-15     Completed Date:  2002-11-18     Revised Date:  2014-10-24    
Medline Journal Info:
Nlm Unique ID:  9604830     Medline TA:  Exp Anim     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  113-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified / genetics*
Antigens, Polyomavirus Transforming / genetics
Cell Differentiation
Cell Line*
Cytological Techniques
Genes, Viral
Genetic Techniques
Mutation
Promoter Regions, Genetic
Rats
Simian virus 40 / genetics
Species Specificity
Temperature
Chemical
Reg. No./Substance:
0/Antigens, Polyomavirus Transforming

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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