Document Detail


Establishment of a tetraploid cell line from mouse H-1 (ES) cells highly polyploidized with demecolcine.
MedLine Citation:
PMID:  17531078     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Establishment of tetraploid ES cells. MATERIALS AND METHODS: Mouse H-1 (ES) cells were polyploidized by demecolcine and released from the drug. RESULTS: A tetraploid cell line (4nH1 cells) was established from mouse H-1 (ES) cells (2nH1 cells) highly polyploidized by treatment with demecolcine. Cell cycle parameters of 4nH1 cells were almost the same as those of 2nH1 cells, suggesting that the rate of DNA synthesis was about twice that of the diploid cells. Mode of chromosome number of 4nH1 cells was 76, about twice that of 2nH1 cells. Cell volume of 4nH1 cells was about twice of that of diploid cells, indicating that 4nH1 cells contained about twice as much total intracellular material as 2nH1 cells. Morphology of the 4nH1 cells was flagstone-like, thus differing from that of the spindle-shaped 2nH1 cells, suggesting that the transformation had occurred during the diploid-tetraploid transition. 4nH1 cells exhibited alkaline phosphatase activity and formed teratocarcinomas, implying that they would be pluripotent. CONCLUSION: A pluripotent tetraploid cell line (4nH1 cells) was established.
Authors:
K Fujikawa-Yamamoto; M Miyagoshi; H Yamagishi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell proliferation     Volume:  40     ISSN:  0960-7722     ISO Abbreviation:  Cell Prolif.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-05-28     Completed Date:  2007-07-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9105195     Medline TA:  Cell Prolif     Country:  England    
Other Details:
Languages:  eng     Pagination:  327-37     Citation Subset:  IM    
Affiliation:
Division of Cell Medicine, Research Institute of Medical Science, Kanazawa Medical University, Uchinada, Ishikawa 920-0293, Japan. fujikawa@kanazawa-med.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents, Phytogenic
Cell Culture Techniques / methods*
Cell Line
Demecolcine
Embryonic Stem Cells / cytology*
Mice
Mice, Inbred C3H
Pluripotent Stem Cells / cytology*
Polyploidy*
Stem Cell Transplantation
Teratocarcinoma
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 477-30-5/Demecolcine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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