Document Detail

Establishment of a tetraploid Meth-A cell line through polyploidization by demecolcine but not by staurosporine, K-252A and paclitaxel.
MedLine Citation:
PMID:  11529879     Owner:  NLM     Status:  MEDLINE    
Polyploid cells are made by DNA reduplication without cell division, however, it is not easy to establish polyploid mammalian cell lines. It is worth studying the difference in cell character between hyperploid and parent cell lines. Meth-A cells were polyploidized by demecolcine, K-252a, staurosporine and paclitaxel. The cell-cycle responses of highly polyploid Meth-A cells after the removal of the drugs were examined by flow cytometry (FCM). Meth-A cells were highly polyploidized by these drugs. The polyploid Meth-A cells gradually decreased in ploidy after the drug release. A tetraploid Meth-A cell line was established only from the demecolcine-induced polyploid Meth-A cells. The duration of G1, S and G2/M phases of the tetraploid cell line were mostly the same as those of the parent diploid cells, except that the G2/M phase was 1.5 h longer. The chromosome number of tetraploid Meth-A cell line was about twice of the diploidy. A tetraploid Meth-A cell line was established.
K Fujikawa-Yamamoto; S Wang; H Yamagishi; C Ohdoi; H Murano; T Ikeda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell proliferation     Volume:  34     ISSN:  0960-7722     ISO Abbreviation:  Cell Prolif.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-08-31     Completed Date:  2001-09-13     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9105195     Medline TA:  Cell Prolif     Country:  England    
Other Details:
Languages:  eng     Pagination:  211-22     Citation Subset:  IM    
Division of Basic Science, Research Institute of Medical Science, Kanazawa Medical University, Ishikawa, Japan.
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MeSH Terms
Antineoplastic Agents, Phytogenic / pharmacology*
Carbazoles / pharmacology
Cell Count
Cell Cycle / drug effects
Cell Division / drug effects
DNA / metabolism
Demecolcine / pharmacology*
Enzyme Inhibitors / pharmacology
Flow Cytometry
Indole Alkaloids
Paclitaxel / pharmacology
Sarcoma / drug therapy*,  genetics,  metabolism,  pathology
Staurosporine / pharmacology
Tumor Cells, Cultured / cytology*,  drug effects*
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Carbazoles; 0/Enzyme Inhibitors; 0/Indole Alkaloids; 33069-62-4/Paclitaxel; 477-30-5/Demecolcine; 62996-74-1/Staurosporine; 9007-49-2/DNA; 97161-97-2/K 252

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