| Establishment of temporomandibular joint puncture technique in rats using in vivo micro-computed tomography (R_mCT®). | |
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MedLine Citation:
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PMID: 20841463 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of the report was to establish puncture techniques for the temporomandibular joint (TMJ) cavity in rats. The experimental sample comprised 30 male Sprague-Dawley rats. Under general anaesthesia the superior joint cavity of the rat was punctured either laterally (lateral puncture technique (LPT), n = 11), anteriorly (anterosuperior puncture technique (ASPT), n = 13) or anteroinferior puncture technique (AIPT), n = 6) using a 27-gauge needle. After the tip of the needle was confirmed by micro-CT (R-mCT®, Rigaku, Tokyo, Japan) located on the mandibular fossa, 0.05 ml of contrast media was injected under micro-CT fluoroscopic guidance. After confirmation that the joint cavity was filled with contrast media, micro-CT imaging was carried out. The puncture for LPT was accurate in 5 of the 11 animals. The ASPT was accurate in all 13 animals. The AIPT punctured 3 of the 6 animals. Furthermore, the ASPT and AIPT demonstrated improved preservation of the needle; it was harder to detach the needle, which led to greater stability. These results suggest that ASPT assisted by R-mCT® is useful for basic research, including drug discovery and pathogenesis of TMJ diseases. |
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Authors:
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S Kameoka; K Matsumoto; Y Kai; Y Yonehara; Y Arai; K Honda |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Dento maxillo facial radiology Volume: 39 ISSN: 0250-832X ISO Abbreviation: Dentomaxillofac Radiol Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-15 Completed Date: 2010-11-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7609576 Medline TA: Dentomaxillofac Radiol Country: England |
Other Details:
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Languages: eng Pagination: 441-5 Citation Subset: D |
Affiliation:
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Department of Oral and Maxillofacial Radiology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo, 101-8310. Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arthrography Contrast Media / administration & dosage Male Needles Punctures / instrumentation, methods* Rats Rats, Sprague-Dawley Temporomandibular Joint / radiography* X-Ray Microtomography* |
| Chemical | |
Reg. No./Substance:
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0/Contrast Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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