Document Detail

Establishment of a reliable extraction method of estrone sulfate from bovine plasma for detection of the peripheral level during the regular estrous cycle by radioimmunoassay.
MedLine Citation:
PMID:  8738860     Owner:  NLM     Status:  MEDLINE    
A simple extraction and assay technique of estrone sulfate in bovine blood was developed with the object of detecting the peripheral level of estrone sulfate in a normal estrous cycle or in early pregnancy. Estrone sulfate in bovine plasma was extracted with a small reversed phase cartridge. The steroid conjugate retained in the cartridge was eluted with 40% (v/v) methanol. Estrone sulfate was separately recovered from other steroids by the stepwise increase in methanol concentration in the elution solvent. The recoveries of estrone sulfate eluted with 40% methanol were more than 90%, irrespective of the applied plasma volume. The concentration measured by radioimmunoassay with the eluent of 40% methanol was consistent for plasma extraction volumes of 0.5-2.0 ml. The change of estrone sulfate in bovine peripheral plasma during the regular estrous cycle was determined with a small reversed phase cartridge for extraction and 40% methanol for elution. The change in estrone sulfate was found to be similar to the change of estrone and estradiol-17 beta. The concentration of estrone sulfate was not higher than that of both estrogens in cattle.
M Hirako; H Takahashi; I Domeki
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Domestic animal endocrinology     Volume:  13     ISSN:  0739-7240     ISO Abbreviation:  Domest. Anim. Endocrinol.     Publication Date:  1996 May 
Date Detail:
Created Date:  1996-10-22     Completed Date:  1996-10-22     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8505191     Medline TA:  Domest Anim Endocrinol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  187-95     Citation Subset:  IM    
Department of Animal Reproduction, National Institue of Animal Industry, Ibaraki, Japan.
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MeSH Terms
Antibodies / blood,  immunology
Cattle / blood*
Chromatography, High Pressure Liquid / methods,  veterinary
Estradiol / blood
Estrogens, Conjugated (USP) / blood,  immunology,  isolation & purification
Estrone / analogs & derivatives*,  blood,  immunology,  isolation & purification
Estrus / blood*
Hydrogen-Ion Concentration
Methanol / chemistry
Progesterone / blood
Radioimmunoassay / methods,  veterinary*
Reg. No./Substance:
0/Antibodies; 0/Estrogens, Conjugated (USP); 481-97-0/estrone sulfate; 50-28-2/Estradiol; 53-16-7/Estrone; 57-83-0/Progesterone; 67-56-1/Methanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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