Document Detail

Establishment of the regeneration system for Vicia faba L.
MedLine Citation:
PMID:  19193964     Owner:  NLM     Status:  MEDLINE    
A reliable regeneration system for faba bean has been difficult to establish and therefore, the genetic improvement of Vicia faba L. was delayed. The paper describes a method of somatic embryo induction in callus of V. faba. Two Egyptian faba bean cultivars 'Giza 2' and '24 Hyto' were used. Callus was induced from epicotyls and shoot tips cultured on MS or Gamborg medium supplemented with 3% sucrose and 0.025% (w/v) for each of ascorbic and citric acid, 0.8% agar and different concentrations of 10 mg/l BAP, 0.5 mg/l of each NAA and 2,4-dichlorophenoxyacetic acid (M1) and 1 mg/l BAP and 0.5 mg/l NAA (M2) . The media with BAP, NAA and 2,4-D were optimal for embryogenic callus induction. Somatic embryos developed after transfer of the callus to 1/2 B5 medium with no plant growth regulators. There were various stages of somatic embryo development present including globular, heart-shaped, torpedo, and cotyledonary stages. Embryos developed into plantlets and plants were regenerated. RAPD analyses were performed to investigate the genetic stability of the regenerated plants obtained from different treatments and different explants. The cultivar Giza 2 exhibited more genetic stability than cultivar 24 Hyto. In conclusion, a regeneration system was established suitable for both gene transformation and the isolation of somaclonal mutants. The regeneration system will be used in order to improve the nutritional value of faba bean.
Shimaa Bahgat; Omer A Shabban; Osama El-Shihy; David A Lightfoot; Hany A El-Shemy
Related Documents :
23684274 - Exogenous treatment with indole-3-acetic acid and salicylic acid alleviates cadmium tox...
15154494 - Aphid (hemiptera: aphididae) resistance in wheat near-isogenic lines.
24912234 - Characterization of bacteria in the rhizosphere soils of polygonum pubescens and their ...
24804624 - Classification of genetic variation for cadmium tolerance in bermudagrass [cynodon dact...
25470674 - Predicting the toxicity of oil-shale industry wastewater by its phenolic composition.
12159954 - Ultrasonic cell disruption of stabilised sludge with subsequent anaerobic digestion.
Publication Detail:
Type:  Journal Article     Date:  2009-02-02
Journal Detail:
Title:  Current issues in molecular biology     Volume:  11 Suppl 1     ISSN:  1467-3037     ISO Abbreviation:  Curr Issues Mol Biol     Publication Date:  2009  
Date Detail:
Created Date:  2009-05-05     Completed Date:  2009-07-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100931761     Medline TA:  Curr Issues Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  i47-54     Citation Subset:  IM    
Faculty of Agriculture Research Park (FARP) and Biochemistry Department, Faculty of Agriculture, University of Cairo, 12613 Giza, Egypt.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cells, Cultured
Culture Media
Plant Growth Regulators / pharmacology,  physiology*
Plant Shoots / embryology,  genetics,  physiology*
Regeneration / physiology*
Tissue Culture Techniques
Vicia faba / embryology,  genetics,  physiology*
Reg. No./Substance:
0/Culture Media; 0/Plant Growth Regulators

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  An account of cloned genes of Methyl-erythritol-4-phosphate pathway of isoprenoid biosynthesis in pl...
Next Document:  Cloning of a novel antifungal promoter from Phaseolus vulgaris and the determination of its activity...