Document Detail

Establishment of a reference panel for the detection of anti-SARS-CoV antibodies.
MedLine Citation:
PMID:  17261372     Owner:  NLM     Status:  MEDLINE    
The immunological assays for detection of antibodies against SARS-CoV were developed in-house and some of them are available commercially. However, the antigens used in these assays differed. In order to validate the reliability of these assays, the standard panel should be established. In this study, we have expressed and purified severe acute respiratory syndrome (SARS) structural proteins and their fragments and developed indirect enzyme-linked immunosorbent assays (ELISAs) that detect antibodies against the SARS N, N(1), N(2), S(1), S(C), S(2), and M proteins as well as the human coronavirus OC43 and 229E N proteins. These assays were used to screen 58 samples from SARS convalescent patients, 40 serial serum specimens from patients at different phases of SARS infection, and 88 plasma specimens from normal blood donors. The samples from normal blood donors were also tested for antibodies against other respiratory virus. The representative samples were chosen to comprise a reference panel of SARS antibodies that may be used for the detection of SARS. The panel is composed of 25 positive samples, 25 negative samples, 7 diluted samples for anti-N antibody, 6 diluted samples for anti-S antibody, and one sample for validating precision. Comparison of detection results with different SARS antibody assays indicated that our panel should differentiate the specificity and sensitivity of different assays.
Chunyu Liu; Xiuhua Li; Chuntao Zhang; Sihong Xu; Yiming Shao; Hui Zhuang; Xiaoyan Che; Yan Qiu; Hongzhang Yin; Defu Li; Youchun Wang
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.     Date:  2007-01-29
Journal Detail:
Title:  Biologicals : journal of the International Association of Biological Standardization     Volume:  35     ISSN:  1045-1056     ISO Abbreviation:  Biologicals     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-18     Completed Date:  2007-08-15     Revised Date:  2007-12-03    
Medline Journal Info:
Nlm Unique ID:  9004494     Medline TA:  Biologicals     Country:  England    
Other Details:
Languages:  eng     Pagination:  203-10     Citation Subset:  IM    
Department of Cell Biology, National Institute for the Control of Pharmaceutical and Biological Products, No. 2 Tiantanxili, Chongwen District, Beijing 100050, PR China.
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MeSH Terms
Antibodies, Viral / blood*
Antigens, Viral / blood
Base Sequence
DNA, Viral / genetics
Enzyme-Linked Immunosorbent Assay / methods,  standards
Genes, Viral
Membrane Glycoproteins / genetics,  immunology
Nucleocapsid Proteins / genetics,  immunology
Reference Standards
SARS Virus / genetics,  immunology*
Severe Acute Respiratory Syndrome / diagnosis,  immunology,  virology
Viral Envelope Proteins / genetics,  immunology
Grant Support
Reg. No./Substance:
0/Antibodies, Viral; 0/Antigens, Viral; 0/DNA, Viral; 0/Membrane Glycoproteins; 0/Nucleocapsid Proteins; 0/Viral Envelope Proteins; 0/nucleocapsid protein, Coronavirus; 107476-75-5/spike glycoprotein, coronavirus

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