Document Detail

Establishment of propagable epithelial cell lines from normal adult rat pancreas.
MedLine Citation:
PMID:  3803447     Owner:  NLM     Status:  MEDLINE    
A method for establishing propagable epithelial cell lines from normal adult rat pancreas is described. Morphological studies showed that these cells were derived from duct epithelial cells. These cells grew equally well in media containing fetal bovine (FBS) or horse serum (HS). Preliminary studies suggested that propagable cultured pancreatic ductal cells during early passages retained some capacity to differentiate into acinar-like cells with the formation of granules resembling zymogen, especially when these cells were cultured on mixed ester cellulose membrane. This supports the concept that pancreatic ductal lining cells represent the 'stem' cells on pancreatic epithelial cells. Propagable pancreatic epithelial cells in long-term cultures will be useful in the histogenetic and mechanistic studies of pancreatic carcinogenesis.
M S Tsao; W P Duguid
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  168     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1987 Feb 
Date Detail:
Created Date:  1987-03-11     Completed Date:  1987-03-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  365-75     Citation Subset:  IM    
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MeSH Terms
Cell Division
Cell Line
Culture Techniques / methods
Epithelial Cells
Epithelium / ultrastructure
Microscopy, Electron
Pancreas / cytology*,  ultrastructure
Rats, Inbred F344
Grant Support

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