Document Detail


Establishment of permanent renal tubule cell lines by infection with the wild-type and a thermosensitive mutant of the simian virus 40.
MedLine Citation:
PMID:  1645927     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study summarizes the properties of rabbit renal tubule cell lines transformed by the simian virus 40 (SV40). By infection with the wild type SV40, it was possible to establish three different cell lines exhibiting the main functions of proximal (RC.SV1), thick ascending limb-distal (RC.SV2), and collecting (RC.SV3) tubules. Another cell line infected with a thermosensitive mutant of SV40 (RC.SVtsA58) was highly sensitive to arginine vasopressin when cells were cultured at the restrictive temperature of 39.5 degrees C, suggesting they derived from principal cells of the collecting tubule.
Authors:
A Vandewalle; P Ronco; R Cassingena
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  American journal of kidney diseases : the official journal of the National Kidney Foundation     Volume:  17     ISSN:  0272-6386     ISO Abbreviation:  Am. J. Kidney Dis.     Publication Date:  1991 Jun 
Date Detail:
Created Date:  1991-07-10     Completed Date:  1991-07-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8110075     Medline TA:  Am J Kidney Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  619-21     Citation Subset:  IM    
Affiliation:
Inserm U.246, CEN Saclay, Gif sur Yvette, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line, Transformed
Cell Transformation, Viral* / physiology
Culture Media
Cytological Techniques
Kidney Tubules / cytology*
Mutation*
Phenotype
Rabbits
Simian virus 40 / pathogenicity*
Temperature
Chemical
Reg. No./Substance:
0/Culture Media

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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