Document Detail


Establishment of an osseous cell line from fetal rat calvaria using an immunocytolytic method of cell selection: characterization of the cell line and of derived clones.
MedLine Citation:
PMID:  2246327     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using selective media and complement-mediated lysis of primary cultures of a fetal rat calvarial cell population, we have developed a cell line (OBCK6) that exhibits osteoblastic characteristics. OBCK6 cells demonstrated enhanced parathyroid hormone (PTH)-stimulated adenylate cyclase activity relative to the primary calvarial population, production of alkaline phosphatase activity and type 1 collagen, and the capacity to form mineralized nodules in unsupplemented medium after prolonged (22-26 day) culture. Two sublines, CFK1 and CFK2, which were isolated by dilution cloning, differed morphologically and with respect to growth rate. CFK1 cells demonstrated high PTH and prostaglandin E2-stimulated adenylate cyclase activity, whereas only low PTH-stimulated activity was observed in CFK2 cells. Retinoic acid and 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] each reduced PTH-stimulated adenylate cyclase activity in both the cell types. Retinoic acid and dexamethasone reduced and 1,25(OH)2D3 enhanced alkaline phosphatase activity in these cells. PTH significantly augmented alkaline phosphatase activity to a much greater extent in CFK1 than in CFK2 cells. Both CFK1 and CFK2 cells expressed type I but type III collagen, and neither expressed osteocalcin. Strong Alcian blue staining of CFK2 cells was suggestive of a cartilaginous phenotype. These three cell lines, therefore, demonstrated discrete characteristics of skeletal cell function and should provide important models for evaluation of mechanisms of mineralization and for control of skeletal cell growth and mesenchymal differentiation in vitro.
Authors:
S M Bernier; J Desjardins; A K Sullivan; D Goltzman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  145     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1991-01-08     Completed Date:  1991-01-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  274-85     Citation Subset:  IM    
Affiliation:
Department of Physiology, McGill University, Montreal, Canada.
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MeSH Terms
Descriptor/Qualifier:
Adenylate Cyclase / metabolism
Alkaline Phosphatase / metabolism
Animals
Bone and Bones / cytology*,  embryology,  metabolism
Cell Division
Cell Line* / cytology,  metabolism
Cell Separation / methods
Clone Cells* / cytology,  metabolism
Collagen / biosynthesis
Fetus / cytology
Glycosaminoglycans / biosynthesis
Rats
Rats, Inbred Strains
Grant Support
ID/Acronym/Agency:
R01 CA37126/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Glycosaminoglycans; 9007-34-5/Collagen; EC 3.1.3.1/Alkaline Phosphatase; EC 4.6.1.1/Adenylate Cyclase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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