| Establishment of a novel immortalized human prostatic epithelial cell line stably expressing androgen receptor and its application for the functional screening of androgen receptor modulators. | |
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MedLine Citation:
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PMID: 19324023 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study, we developed a human prostatic epithelial cell line BPH-1-AR stably expressing AR by lentiviral transduction. Characterization by immunoblot and RT-PCR showed that AR was stably expressed in all representative BPH-1-AR clones. Androgen treatment induced a secretory differentiation phenotype in BPH-1-AR cells but suppressed their cell proliferation. Treatments with AR agonists induced transactivation of a transfected PSA-gene promoter reporter in BPH-1-AR cells, whereas this transactivation was suppressed by an AR antagonist flutamide, indicating that the transduced AR in BPH-1-AR cells was functional. Finally, we utilized BPH-1-AR cells to evaluate the androgenic activities and growth effects of five newly developed non-steroidal compounds. Results showed that these compounds showed androgenic activities and growth-inhibitory effects on BPH-1-AR cells. Our results showed that BPH-1-AR cell line would be a valuable in vitro model for the study of androgen-regulated processes in prostatic epithelial cells and identification of compounds with AR-modulating activities. |
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Authors:
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Shan Yu; Ming-Wei Wang; Xiaoqiang Yao; F L Chan |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-03-24 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 382 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2009 May |
Date Detail:
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Created Date: 2009-04-22 Completed Date: 2009-05-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 756-61 Citation Subset: IM |
Affiliation:
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School of Biomedical Sciences, Faculty of Medicine, Chinese University of Hong Kong, Shatin, Hong Kong, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Androgen Antagonists
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isolation & purification*,
pharmacology Androgens / isolation & purification*, pharmacology Cell Differentiation / drug effects, genetics Cell Line* Drug Evaluation, Preclinical Epithelial Cells / drug effects*, metabolism Humans Male Promoter Regions, Genetic / drug effects Prostate / cytology, drug effects*, metabolism Receptors, Androgen / agonists, antagonists & inhibitors, biosynthesis* Transcriptional Activation Transduction, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Androgen Antagonists; 0/Androgens; 0/Receptors, Androgen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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