Document Detail


Establishment of a novel immortalized human prostatic epithelial cell line stably expressing androgen receptor and its application for the functional screening of androgen receptor modulators.
MedLine Citation:
PMID:  19324023     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we developed a human prostatic epithelial cell line BPH-1-AR stably expressing AR by lentiviral transduction. Characterization by immunoblot and RT-PCR showed that AR was stably expressed in all representative BPH-1-AR clones. Androgen treatment induced a secretory differentiation phenotype in BPH-1-AR cells but suppressed their cell proliferation. Treatments with AR agonists induced transactivation of a transfected PSA-gene promoter reporter in BPH-1-AR cells, whereas this transactivation was suppressed by an AR antagonist flutamide, indicating that the transduced AR in BPH-1-AR cells was functional. Finally, we utilized BPH-1-AR cells to evaluate the androgenic activities and growth effects of five newly developed non-steroidal compounds. Results showed that these compounds showed androgenic activities and growth-inhibitory effects on BPH-1-AR cells. Our results showed that BPH-1-AR cell line would be a valuable in vitro model for the study of androgen-regulated processes in prostatic epithelial cells and identification of compounds with AR-modulating activities.
Authors:
Shan Yu; Ming-Wei Wang; Xiaoqiang Yao; F L Chan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-24
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  382     ISSN:  1090-2104     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-22     Completed Date:  2009-05-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  756-61     Citation Subset:  IM    
Affiliation:
School of Biomedical Sciences, Faculty of Medicine, Chinese University of Hong Kong, Shatin, Hong Kong, China.
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MeSH Terms
Descriptor/Qualifier:
Androgen Antagonists / isolation & purification*,  pharmacology
Androgens / isolation & purification*,  pharmacology
Cell Differentiation / drug effects,  genetics
Cell Line*
Drug Evaluation, Preclinical
Epithelial Cells / drug effects*,  metabolism
Humans
Male
Promoter Regions, Genetic / drug effects
Prostate / cytology,  drug effects*,  metabolism
Receptors, Androgen / agonists,  antagonists & inhibitors,  biosynthesis*
Transcriptional Activation
Transduction, Genetic
Chemical
Reg. No./Substance:
0/Androgen Antagonists; 0/Androgens; 0/Receptors, Androgen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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