| Establishment of a novel human myeloid leukemia cell line, AMU-AML1, carrying t(12;22)(p13;q11) without chimeric MN1-TEL and with high expression of MN1. | |
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MedLine Citation:
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PMID: 21965128 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In this study, we established and analyzed a novel human myeloid leukemia cell line, AMU-AML1, from a patient with acute myeloid leukemia with multilineage dysplasia before the initiation of chemotherapy. AMU-AML1 cells were positive for CD13, CD33, CD117, and HLA-DR by flow cytometry analysis and showed a single chromosomal abnormality, 46, XY, t(12;22)(p13;q11.2), by G-banding and spectral karyotyping. Fluorescent in situ hybridization analysis indicated that the chromosomal breakpoint in band 12p13 was in the sequence from the 5' untranslated region to intron 1 of TEL and that the chromosomal breakpoint in band 22q11 was in the 3' untranslated region of MN1. The chimeric transcript and protein of MN1-TEL could not be detected by reverse-transcriptase polymerase chain reaction or Western blot analysis. However, the MN1 gene was amplified to three copies detected by array comparative genomic hybridization analysis, and the expression levels of the MN1 transcript and protein were high in AMU-AML1 cells when compared with other cell lines with t(12;22)(p13;q11-12). Our data showed that AMU-AML1 cells contain t(12;22)(p13;q11.2) without chimeric fusion of MN1 and TEL. The AMU-AML1 cells gained MN1 copies and had high expression levels of MN1. Thus, the AMU-AML1 cell line is useful for studying the biological consequences of t(12;22)(p13;q11.2) lacking chimeric MN1-TEL. © 2011 Wiley-Liss, Inc. |
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Authors:
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Mayuko Gotou; Ichiro Hanamura; Hisao Nagoshi; Motohiro Wakabayashi; Natsumi Sakamoto; Norikazu Tsunekawa; Tomohiro Horio; Mineaki Goto; Shohei Mizuno; Miyuki Takahashi; Kazuto Suganuma; Hidesuke Yamamoto; Akihito Hiramatsu; Masaya Watarai; Masato Shikami; Akira Imamura; Hidetsugu Mihara; Tomohiko Taki; Hiroshi Miwa; Masafumi Taniwaki; Masakazu Nitta |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-2 |
Journal Detail:
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Title: Genes, chromosomes & cancer Volume: - ISSN: 1098-2264 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-3 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9007329 Medline TA: Genes Chromosomes Cancer Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Wiley-Liss, Inc. |
Affiliation:
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Division of Hematology, Department of Internal Medicine, Aichi Medical University, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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