Document Detail

Establishment of a novel MALT lymphoma cell line, ma-1, from a patient with t(14;18)(q32;q21)-positive Helicobacter pylori-independent gastric MALT lymphoma.
MedLine Citation:
PMID:  21837708     Owner:  NLM     Status:  MEDLINE    
Although t(11;18)(q21;q21), t(1;14)(p22;q32), and a few other genetic mutations are specific markers for the Helicobacter pylori (HP)-independent status of gastric mucosa-associated lymphoid tissue (MALT) lymphoma, the molecular mechanisms responsible for HP-independence of gastric MALT lymphoma without such translocations and mutations remain uncharacterized. In the present study, we describe the establishment and characterization of a novel MALT lymphoma cell line, MA-1, which was derived from a gastric MALT lymphoma which was negative for both t(11;18)(q21;q21) and t(1;14)(p22;q32); the patient had failed HP eradication therapy and chemotherapy. The cell morphology and the immunophenotype of this cell line were similar to that of the original gastric MALT lymphoma. Comparative genomic hybridization analysis showed no significant gene copy number changes. Spectral karyotyping displayed a near-diploid chromosome content (48 < 2n>XY), with at least 13 chromosome structural abnormalities. Furthermore, fluorescence in situ hybridization analyses disclosed the existence of three sub-clones, characterized by t(14;18)(q32;q21)/IGH-BCL2, t(14;18)(q32;q21)/IGH-MALT1, and the presence of both chromosomal translocations in the same cell, respectively; whereas amplification of the genes CRAD9, TRAF2, and BCL10 were not found. In conclusion, we have established the first human gastric MALT lymphoma cell line, which is characterized by unusual and complex chromosome translocations and will be useful to explore further the molecular mechanisms of HP-independence in gastric MALT lymphoma.
Sung-Hsin Kuo; Wen-Hui Weng; Zi-Hua Chen; Ping-Ning Hsu; Ming-Shiang Wu; Chung-Wu Lin; Huei-Jiuan Jeng; Kun-Huei Yeh; Hui-Jen Tsai; Li-Tzong Chen; Ann-Lii Cheng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-08-11
Journal Detail:
Title:  Genes, chromosomes & cancer     Volume:  50     ISSN:  1098-2264     ISO Abbreviation:  Genes Chromosomes Cancer     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-09-12     Completed Date:  2012-03-15     Revised Date:  2013-10-10    
Medline Journal Info:
Nlm Unique ID:  9007329     Medline TA:  Genes Chromosomes Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  908-21     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Wiley-Liss, Inc.
Department of Oncology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei.
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MeSH Terms
Cell Line, Tumor*
Chromosomes, Human, Pair 14
Chromosomes, Human, Pair 18
Comparative Genomic Hybridization
Flow Cytometry
Gene Dosage
Helicobacter Infections / pathology
Helicobacter pylori
In Situ Hybridization, Fluorescence
Lymphoma, B-Cell, Marginal Zone / genetics,  metabolism,  microbiology,  pathology*
Lymphoma, Non-Hodgkin / genetics,  metabolism,  microbiology,  pathology*
Microsatellite Repeats
Middle Aged
Spectral Karyotyping
Stomach Neoplasms / genetics,  metabolism,  microbiology,  pathology*
Translocation, Genetic
Comment In:
Genes Chromosomes Cancer. 2013 Jun;52(6):593-4   [PMID:  23508748 ]
Genes Chromosomes Cancer. 2013 Jun;52(6):591-2   [PMID:  23508779 ]
Genes Chromosomes Cancer. 2013 Oct;52(10):986-8   [PMID:  23907998 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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