| Establishment of mutant murine mammary carcinoma FM3A cell strains transformed with the herpes simplex virus type 2 thymidine kinase gene. | |
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MedLine Citation:
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PMID: 3021345 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To establish cell systems appropriate for investigating the mode of action of antiherpetic nucleoside analogues, mutant cell strains were constructed from murine mammary carcinoma FM3A cells, which were deficient in TK, but were transformed with a recombinant plasmid DNA containing the HSV-2 TK gene. The transformed cells incorporated the viral DNA, expressed viral TK activity and showed unusually high sensitivity to the cytostatic action of the antiherpetic nucleoside analogues ACV and IVDU, both of which were only weakly inhibitory to the growth of the parent cells. Curiously, the FM3A cell strains transformed with HSV-2 TK gene showed a higher sensitivity to ACV and IVDU than the previously established cell line transformed with HSV-1 TK gene. This contrasts with the inhibitory effects of ACV and IVDU on acute HSV infection, since HSV-2 infection is slightly or considerably less susceptible than HSV-1 infection to inhibition by ACV or IVDU, respectively. |
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Authors:
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K Shimizu; L Ren; D Ayusawa; T Seno; J Balzarini; E De Clercq |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell structure and function Volume: 11 ISSN: 0386-7196 ISO Abbreviation: Cell Struct. Funct. Publication Date: 1986 Sep |
Date Detail:
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Created Date: 1986-12-17 Completed Date: 1986-12-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7608465 Medline TA: Cell Struct Funct Country: JAPAN |
Other Details:
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Languages: eng Pagination: 295-301 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acyclovir
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pharmacology Animals Cell Line Cell Transformation, Viral* Cloning, Molecular / methods* Genes, Viral* Idoxuridine / analogs & derivatives, pharmacology Mammary Neoplasms, Experimental / genetics, pathology* Mice Mutation Simplexvirus / enzymology, genetics* Thymidine Kinase / genetics* |
| Chemical | |
Reg. No./Substance:
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54-42-2/Idoxuridine; 59277-89-3/Acyclovir; 87727-68-2/5-(2-iodovinyl)-2'-deoxyuridine; EC 2.7.1.21/Thymidine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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