Document Detail


Establishment of an insect cell clone that harbours a partial baculoviral genome and is resistant to homologous virus infection.
MedLine Citation:
PMID:  19675188     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
After serially undiluted passage of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV), persistently infected Se301 cells were established. A cell strain, in which no polyhedra or viral particles were observed, was cloned and designated P8-Se301-C1. The P8-Se301-C1 cells are morphologically similar to but grow slower than Se301 cells and they can homologously interfere with SeMNPV. PCR analysis showed that SeMNPV ie-0 and polyhedrin genes were present but DNA polymerase and orf67 genes were absent in P8-Se301-C1, suggesting that the cells harbour incomplete SeMNPV genomes. Dot-blot analysis demonstrated that 0.32+/-0.16 ng SeMNPV DNA was present in 1.25 x 10(5) P8-Se301-C1 cells. A quantitative real-time PCR assay showed that there were 13.2+/-4.3 copies of the SeMNPV polyhedrin gene in each cell. Nested RT-PCR demonstrated the presence of SeMNPV polyhedrin transcripts in P8-Se301-C1 cells. The fact that P8-Se301-C1 cells carry low levels of partial viral genome but do not produce viral progeny suggests a latent-like viral infection in the cells.
Authors:
Qingbei Weng; Kai Yang; Wei Xiao; Meijin Yuan; Wenjun Zhang; Yi Pang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-12
Journal Detail:
Title:  The Journal of general virology     Volume:  90     ISSN:  1465-2099     ISO Abbreviation:  J. Gen. Virol.     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-11     Completed Date:  2009-12-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0077340     Medline TA:  J Gen Virol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2871-6     Citation Subset:  IM    
Affiliation:
State Key Laboratory of Biocontrol, Sun Yat-sen University, Guangzhou 510275, PR China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Clone Cells
Genome, Viral
Nucleopolyhedrovirus / classification,  genetics,  pathogenicity*,  physiology*
Reverse Transcriptase Polymerase Chain Reaction
Serial Passage
Spodoptera / virology*
Viral Structural Proteins / genetics,  metabolism
Virus Latency*
Chemical
Reg. No./Substance:
0/Viral Structural Proteins; 0/polyhedrin protein, Nucleopolyhedrovirus

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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