Document Detail


Establishment of an immunodeficient alcohol mouse model to study the effects of alcohol on human cells in vivo.
MedLine Citation:
PMID:  23036211     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: The effects of alcohol (ethanol) are well documented and contribute to significant health problems and financial burden on the health care system. Several mouse models have been described that facilitate studies of the effects of alcohol on the mouse immune system. Our goal was to establish a chronic alcohol mouse model using the immunodeficient NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mouse. This severely immunodeficient model has previously been shown to allow efficient engraftment of human hematopoietic repopulating cells and cancer cells, thereby facilitating diverse studies on human hematopoiesis, immune cell function, and oncogenesis in vivo.
METHOD: NSG mice were provided ethanol in their drinking water as the only available fluid, starting at 5% weight/volume (w/v) and subsequently were increased to 10%, 15%, and 20% w/v. Mice were then maintained at 20% w/v, a level that models chronic alcohol use in humans. Alcohol consumption and weight were monitored.
RESULTS: NSG mice readily consumed alcohol throughout the study and showed no adverse effects. No significant difference between group mean weights was identified the day before increasing the ethanol dose or at the end of 5 weeks at 20% w/v (p > .28). While the mice were maintained at 20% w/v ethanol, the mean daily ethanol intake was 27.2 g/kg of body weight, 32% of caloric intake.
CONCLUSIONS: Here we have established a chronic alcohol mouse model using the powerful immunodeficient NSG mouse. This model should allow for novel studies on the effects of alcohol on engrafted human cells, including studies on the effects of alcohol on hematopoiesis, immunity, and cancer.
Authors:
John Powers; Hui Zhang; Logan Battrell; Gary G Meadows; Grant D Trobridge
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of studies on alcohol and drugs     Volume:  73     ISSN:  1938-4114     ISO Abbreviation:  J Stud Alcohol Drugs     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-05     Completed Date:  2013-01-11     Revised Date:  2013-11-05    
Medline Journal Info:
Nlm Unique ID:  101295847     Medline TA:  J Stud Alcohol Drugs     Country:  United States    
Other Details:
Languages:  eng     Pagination:  933-7     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical Sciences, Washington State University, Pullman, Washington.
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MeSH Terms
Descriptor/Qualifier:
Alcohol Drinking / physiopathology*,  psychology*
Alcoholism / physiopathology,  psychology*
Animals
Body Weight / drug effects,  physiology
Disease Models, Animal*
Eating / drug effects,  physiology
Energy Intake / drug effects,  physiology
Female
Mice
Mice, Mutant Strains / immunology,  physiology,  psychology*
Grant Support
ID/Acronym/Agency:
K05AA017149/AA/NIAAA NIH HHS; R01AA07293/AA/NIAAA NIH HHS
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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