| Establishment of immortalized clonal cells derived from periodontal ligament cells by induction of the hTERT gene. | |
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MedLine Citation:
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PMID: 16415561 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Since the periodontal ligament (PDL) contains a heterogeneous cell population, it is challenging to identify all cell types within the tissue and to determine whether they function alone to produce tissue components or interact with other cell types. Further, it is difficult to isolate and expand single cell clones from PDL cells, as normal cells have a limited life span and are phenotypically unstable. In the present study, we inserted the human telomerase reverse transcriptase (hTERT) gene, which encodes the catalytic subunit of the telomerase holoenzyme, into normal human periodontal ligament (HPL) cells and successfully obtained single cell clones. Expression of the inserted gene and telomerase activity in each of the clones was confirmed. Unlike the original HPL cells, at the end of the study (day 120), clone populations continued to actively double without phenotypic alteration. Osteogenic characteristics were present in some but not all clones. In conclusion, immortalization of HPL cells was successfully accomplished by transduction with the hTERT gene. This is the first report of immortalization of different cell types derived from PDL. |
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Authors:
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Tomohisa Fujita; Yoko Otsuka-Tanaka; Hidetoshi Tahara; Toshinori Ide; Yoshimitsu Abiko; Jun-ichi Mega |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of oral science Volume: 47 ISSN: 1343-4934 ISO Abbreviation: J Oral Sci Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2006-01-17 Completed Date: 2006-03-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9808942 Medline TA: J Oral Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 177-84 Citation Subset: D; IM |
Affiliation:
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Department of Dentistry for the Disabled, Nihon University School of Dentistry at Matsudo, Japan. ftom@mascat.nihon-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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analysis Calcium Phosphates / analysis Catalytic Domain / genetics* Cell Proliferation Clone Cells / pathology DNA-Binding Proteins / genetics* Gene Amplification Gene Expression Regulation, Enzymologic / genetics Humans Osteogenesis / genetics Periodontal Ligament / pathology* Phenotype Telomerase / genetics* Telomere / genetics Time Factors Transduction, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Calcium Phosphates; 0/DNA-Binding Proteins; EC 2.7.7.49/Telomerase; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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