| Establishment of immortal swine kidney epithelial cells. | |
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MedLine Citation:
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PMID: 16621759 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Using normal swine kidney epithelial (SKE) cells that were shown to be senescent at passages 12 to 14, we have established one lifespan-extended cell line and two lifespan-extended cell lines by exogenous introduction of the human catalytic subunit of telomerase (hTERT) and simian virus 40 large T-antigen (SV40LT), all of which maintain epithelial morphology and express cytokeratin, a marker of epithelial cells. SV40LT- and hTERT-transduced immortal cell lines appeared to be smaller and exhibited more uniform morphology relative to primary and spontaneously immortalized SKE cells. We determined the in vitro lifespan of primary SKE cells using a standard 3T6 protocol. There were two steps of the proliferation barrier at 12 and 20, in which a majority of primary SKE cells appeared enlarged, flattened, vacuolated, and ss-galactosidase-positive, all phenotypical characteristics of senescent cells. Lifespan-extended SKE cells were eventually established from most of the cellular foci, which is indicative of spontaneous cellular conversion at passage 23. Beyond passage 25, the rate of population doubling of the established cells gradually increased. At passage 30, immortal cell lines grew faster than primary counterpart cells in 10% FBS-DMEM culture conditions, and only SV40LT-transduced immortal cells grew faster than primary and other SKE immortal cells in 0.5% FBS-DMEM. These lifespan-extended SKE cell lines failed to grow in an anchorage-independent manner in soft-agar dishes. Hence, three immortalized swine kidney epithelial cells that are not transformed would be valuable biological tools for virus propagation and basic kidney epithelial cell research. |
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Authors:
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Sungwook Kwak; Ji-Eun Jung; Xun Jin; Sun-Myung Kim; Tae-Kyung Kim; Joong-Seob Lee; Soo-Yeon Lee; Xumin Pian; Seungkwon You; Hyunggee Kim; Yun-Jaie Choi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Animal biotechnology Volume: 17 ISSN: 1049-5398 ISO Abbreviation: Anim. Biotechnol. Publication Date: 2006 |
Date Detail:
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Created Date: 2006-04-19 Completed Date: 2006-06-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9011409 Medline TA: Anim Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 51-8 Citation Subset: IM |
Affiliation:
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Laboratory of Cell Growth and Function Regulation, Division of Bioscience and Technology, College of Life and Environmental Sciences, Korea University, Seoul, Korea. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Culture Techniques Cell Line, Transformed* DNA-Binding Proteins / genetics Epithelial Cells / cytology*, enzymology Kidney / cytology*, enzymology Simian virus 40 / genetics Swine* Telomerase / genetics Transformation, Genetic |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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