Document Detail

Establishment of a human hepatocyte line that supports in vitro development of the exo-erythrocytic stages of the malaria parasites Plasmodium falciparum and P. vivax.
MedLine Citation:
PMID:  16687667     Owner:  NLM     Status:  MEDLINE    
Our understanding of the biology of malaria parasite liver stages is limited because of the lack of efficient in vitro systems that support the exo-erythrocytic (EE) development of the parasite. We report the development of a new hepatocyte line (HC-04) from normal human liver cells. The HC-04 cells have proliferated in hormone-free medium for more than 200 passages. The cells were hyperdiploid, resembled liver parenchymal cells, and synthesized major liver-specific proteins and enzymes. Using Plasmodium falciparum and P. vivax sporozoites harvested from salivary glands of infected mosquitoes, we showed that HC-04 cells supported the complete EE development of these two most prevalent human malaria parasites. The EE parasites attained full maturation as shown by their infectivity to human erythrocytes. The infection rates of the liver cells were estimated to be 0.066% and 0.041% for P. falciparum and P. vivax, respectively. As the first human hepatocyte line known to support complete EE development of both P. falciparum and P. vivax, HC-04 will provide an experimental model that can be used for studying the biology of liver stage malaria parasites.
Jetsumon Sattabongkot; Nongnuch Yimamnuaychoke; Surasak Leelaudomlipi; Maneerat Rasameesoraj; Rachaneeporn Jenwithisuk; Russell E Coleman; Rachanee Udomsangpetch; Liwang Cui; Thomas G Brewer
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The American journal of tropical medicine and hygiene     Volume:  74     ISSN:  0002-9637     ISO Abbreviation:  Am. J. Trop. Med. Hyg.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-11     Completed Date:  2006-06-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370507     Medline TA:  Am J Trop Med Hyg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  708-15     Citation Subset:  AIM; IM    
Department of Entomology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand.
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MeSH Terms
Albumins / metabolism
Cells, Cultured
Hepatocytes / enzymology,  metabolism,  parasitology*
Malaria, Falciparum / parasitology*
Malaria, Vivax / parasitology*
Plasmodium falciparum / physiology*
Plasmodium vivax / physiology*
Transferrin / metabolism
alpha-Fetoproteins / metabolism
Grant Support
1D43 TW000657/TW/FIC NIH HHS
Reg. No./Substance:
0/Albumins; 0/alpha-Fetoproteins; 11096-37-0/Transferrin
Comment In:
Am J Trop Med Hyg. 2006 May;74(5):706-7   [PMID:  16687666 ]

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