Document Detail


Establishment of human gastric epithelial (HGE) cell lines exhibiting barrier function, progenitor, and prezymogenic characteristics.
MedLine Citation:
PMID:  15389599     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The unavailability of human cell lines representative of the gastric glandular epithelium while able to form a functional barrier restricts the application of a cell culture approach to the field of gastric epithelial physiology. In the current study, we have characterized new non-transfected clones isolated from gastric carcinoma cell lines known to express functional markers of the human gastric mucosa (J Cell Biochem 2001;81:241). Twenty-one clones exhibiting epithelial-type junctions (renamed HGE cell lines) were isolated from NCI-N87 (ATCC CRL 5822), whereas only squamous cell lines could be generated from other native strains. Of these 21 clones, HGE-17 and HGE-20 formed dense coherent monolayers and displayed true epithelial phenotype. E-cadherin and ZO-1 proteins were consistently localized at the periphery of all cells which also generated transepithelial electrical resistance. Moreover, growth factors known to be trophic for the gastric mucosa were able to stimulate mitogenesis at subconfluence. HGE-17 exhibited a poorly differentiated precursor-like status and responded strongly to EGF/TGFalpha treatment in restitution assays. HGE-20 cells, on the other hand, exhibited a higher degree of differentiation at the ultrastructural level as well as higher gastric lipase and pepsinogen levels. These latter zymogens were compartmentalized into granules which also contained mucin-6 (MUC6, prezymogenic-like status). Exogenous hormones, i.e., 1 mug/ml hydrocortisone and 5 microM retinoic acid, significantly increased enzyme levels in HGE-20. In conclusion, HGE-17 and HGE-20 represent the first human gastric cell lines with true epithelial characteristics, opening a venue to important applications for the study of re-epithelization, permeability, and regulation of digestive functions in the context of gastric physiology and pathology.
Authors:
Pierre Chailler; Daniel Ménard
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  202     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2004-11-03     Completed Date:  2005-01-25     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  263-74     Citation Subset:  IM    
Copyright Information:
2005 Wiley-Liss, Inc.
Affiliation:
CIHR Group on Functional Development and Physiopathology of the Digestive Tract, Department of Anatomy and Cell Biology, Faculty of Medicine, Université de Sherbrooke, Sherbrooke (Québec), Canada.
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MeSH Terms
Descriptor/Qualifier:
Artificial Organs / trends*
Cadherins / metabolism
Cell Culture Techniques / methods*
Cell Differentiation / drug effects,  physiology
Cell Line
Cell Line, Tumor
Cell Proliferation / drug effects
Electric Impedance
Enzyme Precursors / biosynthesis*
Epithelial Cells / physiology*,  ultrastructure
Gastric Mucins / metabolism
Gastric Mucosa / physiology*,  ultrastructure
Growth Substances / pharmacology
Humans
Hydrocortisone / pharmacology
Lipase / metabolism
Membrane Proteins / metabolism
Microscopy, Electron, Transmission
Organogenesis / physiology
Pepsinogen A / metabolism
Phosphoproteins / metabolism
Secretory Vesicles / metabolism,  ultrastructure
Stem Cells / physiology*,  ultrastructure
Tretinoin / pharmacology
Chemical
Reg. No./Substance:
0/Cadherins; 0/Enzyme Precursors; 0/Gastric Mucins; 0/Growth Substances; 0/Membrane Proteins; 0/Phosphoproteins; 0/zonula occludens-1 protein; 302-79-4/Tretinoin; 50-23-7/Hydrocortisone; 9001-10-9/Pepsinogen A; EC 3.1.1.3/Lipase

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