Document Detail


Establishment of a highly metastatic tongue squamous cell carcinoma cell line from New Zealand White rabbit.
MedLine Citation:
PMID:  18691699     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: Prior to this study, the widely used tongue squamous cell carcinoma cell lines could only initiate tumours in immunodeficient mice, which greatly delayed studies on immune function during carcinogenesis. This study established a new tongue squamous cell carcinoma cell line named 'RSCC-1', which can initiate tumours in both immunocompetent rabbits and immunodeficient nude mice and has high metastatic ability. DESIGN: Primary tongue cancer was induced by DMBA and local mechanical stimulation in New Zealand White rabbits. The induced cancer was serially transplanted into homogeneous rabbits to establish transplanted models. At the same time, cancer samples were collected, cultured and passaged in vitro. Finally, a cell line named 'RSCC-1' was established. Its growth behaviour, cell cycle distribution and tumourigenicity in rabbits and nude mice were investigated. RESULTS: RSCC-1 cells were cultured continuously in vitro for 19 months (165 passages). They contain between 54 and 196 chromosomes, with a modal number of 75. Tumourigenicity rates were 100% in both homogeneous rabbits and nude mice, with 20% lung metastasis and 50% cervical lymph node metastasis in homogeneous rabbits. CONCLUSION: RSCC-1 is a poorly differentiated, highly malignant rabbit tongue squamous cell carcinoma cell line. Its behaviour in the inoculated animal model closely resembles human tongue cancer, and could metastasize to local lymph nodes and remote organs.
Authors:
Jin Wulong; Liang Zhou; Zhou Xiaojian; Tian Jie; Guo Huilin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-08-08
Journal Detail:
Title:  Archives of oral biology     Volume:  53     ISSN:  1879-1506     ISO Abbreviation:  Arch. Oral Biol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-09-16     Completed Date:  2009-04-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0116711     Medline TA:  Arch Oral Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1084-90     Citation Subset:  D; IM    
Affiliation:
Department of Head and Neck Surgery, Ear Nose and Throat Hospital, Fudan University, Shanghai 200031, China.
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MeSH Terms
Descriptor/Qualifier:
9,10-Dimethyl-1,2-benzanthracene
Animals
Carcinoma, Squamous Cell / chemically induced,  pathology,  secondary*,  ultrastructure
Cell Cycle
Cell Division
Cell Line, Tumor / pathology*,  ultrastructure
Disease Models, Animal
Mice
Mice, Nude
Microscopy, Electron
Neoplasm Transplantation
Rabbits
Tongue Neoplasms / chemically induced,  pathology*,  ultrastructure
Chemical
Reg. No./Substance:
57-97-6/9,10-Dimethyl-1,2-benzanthracene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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