| Establishment of hepatocyte extraction combined with HPLC(HE-HPLC) and application in analysis of active components in the fruits of Gardenia jasminoides extract | |
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MedLine Citation:
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PMID: 19459310 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To screen effective principles from traditional Chinese medicine, a method named hepatocyte extraction combined with HPLC (HE-HPLC) was establish in this study. The active principles in the fruits of Gardenia jasminoides Ellis extract were combined with the hepatocytes under imitated physiological environments. Then the unattached substances were washed off by PBS with pH 7.4. After that the conjugated compounds were eluted by PBS with pH 4.0. These compounds released from target sites were collected and handled through SPE to be condensed, and analyzed by HPLC. The results indicated that two characteristic active compounds in the fruits of G. jasminoides extract binded to the hepatocytes. One of them is geniposide. The other is continued to be identified. It is showed that active principles which could bind to hepotocyte (through receptors, Channels, enzymes, etc.) could be detected, at least partly, by HE-HPLC analysis. There was a significant correlation between the retention properties of the active compounds which was obtained by HE-HPLC and their pharmacological effects on hepotocytes. |
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Authors:
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Min Hong; Hongyu Ma; Quan Zhu |
Publication Detail:
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Type: English Abstract; Journal Article |
Journal Detail:
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Title: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica Volume: 34 ISSN: 1001-5302 ISO Abbreviation: Zhongguo Zhong Yao Za Zhi Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-05-22 Completed Date: 2009-06-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8913656 Medline TA: Zhongguo Zhong Yao Za Zhi Country: China |
Other Details:
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Languages: chi Pagination: 450-3 Citation Subset: IM |
Affiliation:
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National Standard Laboratory of Traditional Chinese Medicine Pharmacology, Nanjing University of Chinese Medicine, Nanjing 210029, China. hongmin72@hotmail.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, High Pressure Liquid / methods Drugs, Chinese Herbal / analysis* Fruit / chemistry Gardenia / chemistry* Hepatocytes / drug effects*, metabolism Iridoids / pharmacology* Phytotherapy Plant Extracts / pharmacology* Rats |
| Chemical | |
Reg. No./Substance:
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0/Drugs, Chinese Herbal; 0/Iridoids; 0/Plant Extracts; 24512-63-8/geniposide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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