Document Detail

Establishment and growth kinetics of the mutant Ehrlich-Lettré ascites cell strain HD33 in permanent suspension culture.
MedLine Citation:
PMID:  3719659     Owner:  NLM     Status:  MEDLINE    
Cells of a mutant in vivo subline of the Ehrlich-Lettré mouse ascites tumour (ELAT) were converted to growth in suspension culture. Kinetic analysis revealed the selective character of the conversion process; without a detectable adaptation period, a fraction of about 2 X 10(-5) of the explanted cells continued to grow in vitro. The resulting, mutant Ehrlich-Lettré ascites cell strain was designated HD33 and propagated uninterruptedly from 1974 on. The corresponding in vivo ELAT subline HD33 was derived from the HD33 ascites cell strain by intraperitoneal retransplantation. In HD33 cell suspension cultures, the population doubling time, the average intermitotic interval, as determined by videomonitoring, and the average duration of the cell cycle, as determined from percentage of labelled mitoses (PLM) data, were all measured at 15 hr. Cell loss and quiescent compartments were insignificant. The duration of the G1 phase was effectively zero. Both PLM data and [3H]/[14C] thymidine double-labelling measurements revealed an S-phase duration of between 11 and 12 hr. The G2 phase lasted 3-5 hr. The HD33 strain differs from comparable suspension strains of wild-type Ehrlich ascites cells in the insignificant role of density-dependent inhibition in growth, and the striking prolongation of the S phase which is associated with an excessive, cytoplasmic storage of glycogen by the mutant cells.
C Granzow; V Granzow; L Edler; W Sauer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell and tissue kinetics     Volume:  19     ISSN:  0008-8730     ISO Abbreviation:  Cell Tissue Kinet     Publication Date:  1986 May 
Date Detail:
Created Date:  1986-08-21     Completed Date:  1986-08-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0174107     Medline TA:  Cell Tissue Kinet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  245-55     Citation Subset:  IM    
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MeSH Terms
Carbon Radioisotopes
Carcinoma, Ehrlich Tumor / genetics,  pathology*
Cell Cycle*
Cell Division
Cell Line
Cell Survival
Mitotic Index
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Suspensions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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