| Establishment of a feline astrocyte-derived cell line (G355-5 cells) expressing feline CD134 and a rapid quantitative assay for T-lymphotropic feline immunodeficiency viruses. | |
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MedLine Citation:
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PMID: 18584886 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Few laboratory strains of feline immunodeficiency virus (FIV) can infect Crandell feline kidney cells (an epithelial-type of cells), however, most primary isolates are T-lymphotropic. T-lymphotropic FIV requires both feline CD134 (an activation marker of helper T-lymphocytes) and CXCR4 (a chemokine receptor) in infection as primary and secondary receptors, respectively. Using feline T-lymphoblastoid cell lines, titration of primary FIV isolates was carried out, however the titration assay was laborious and time-consuming. In this study, using G355-5 cells (a feline astrocyte-derived cell line) transduced with a cDNA of feline CD134 as target cells, an assay system was developed to quantitate primary FIV isolates. With a previous method using a feline T-lymphoblastoid cell line (MYA-1 cells) highly sensitive to FIV, it took 12 days to complete the assay, however, it took only 2 days with the new method. The FIV-infected cells became in a state of persistent infection, producing a large amount of FIV, indicating that the cells will be useful for propagation of T-lymphotropic FIV strains. |
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Authors:
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Mieko Ishikawa; Masaya Okada; Kenji Baba; Takayuki Shojima; Masayuki Shimojima; Tomoyuki Miura; Takayuki Miyazawa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-06-27 |
Journal Detail:
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Title: Journal of virological methods Volume: 151 ISSN: 0166-0934 ISO Abbreviation: J. Virol. Methods Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-07-14 Completed Date: 2008-11-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8005839 Medline TA: J Virol Methods Country: Netherlands |
Other Details:
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Languages: eng Pagination: 242-8 Citation Subset: IM |
Affiliation:
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Laboratory of Viral Pathogenesis, Center for Emerging Virus Research, Institute for Virus Research, Kyoto University, 53 Shogoin-Kawaracho, Sakyo-ku, Kyoto 606-8507, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Astrocytes / cytology, immunology*, virology* Cats Cell Line Flow Cytometry Immunodeficiency Virus, Feline / growth & development*, isolation & purification Receptors, OX40 / analysis* |
| Chemical | |
Reg. No./Substance:
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0/Receptors, OX40 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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