| Establishment of the expression system for studying the function of active caspase-3 in zebrafish. | |
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MedLine Citation:
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PMID: 18080784 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Caspase-3, a key molecule in apoptosis, has been extensively studied in cell culture system; however, it has been less well characterized in vivo because certain mediators are required for the proteolytic activation of effector caspases, including caspase-3. In this study, various forms of caspase-3 with the C-terminal GFP tag were inserted into the pCS2+ plasmid, and the expression patterns of caspase-3 proteins were characterized in a zebrafish model system using microinjection of nucleic acids into zebrafish embryos. We have verified that active caspase-3 was generated by its autocatalytic activity under the condition of caspase-2 prodomain (C2P)-caspase-3-GFP overexpression, indicating that the C2P domain is crucial for the activation of caspase-3. We also confirmed that the C2P domain plays an important role in regulating the nuclear localization of the C2P-caspase-3 chimeric protein. We used this expression system to establish an animal model system suitable for the investigation of the functional characteristics of caspase-3 in vivo. Thus, our study provides a useful and specific tool for investigating the molecular mechanisms by which active caspase-3 regulates apoptosis during embryonic development. |
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Authors:
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Ho-Young Kim; Goo-Young Kim; Sang-Soo Kim; Min-Kyung Nam; Hyangshuk Rhim |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-13 |
Journal Detail:
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Title: Molecular biology reports Volume: 36 ISSN: 1573-4978 ISO Abbreviation: Mol. Biol. Rep. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2008-12-30 Completed Date: 2009-08-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0403234 Medline TA: Mol Biol Rep Country: Netherlands |
Other Details:
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Languages: eng Pagination: 405-13 Citation Subset: IM |
Affiliation:
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Department of Biomedical Sciences, The Catholic University of Korea, Seoul 137-701, Korea. hrhim@catholic.ac.kr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Active Transport, Cell Nucleus Animals Caspase 3 / genetics* Enzyme Activation Green Fluorescent Proteins / administration & dosage Microinjections Models, Animal* Plasmids / administration & dosage Transfection / methods* Zebrafish |
| Chemical | |
Reg. No./Substance:
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147336-22-9/Green Fluorescent Proteins; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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