| Establishment of an epidermal growth factor-dependent, multipotent neural precursor cell line. | |
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MedLine Citation:
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PMID: 9719419 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have established a multipotent clonal cell line, named MEB5, from embryonic mouse forebrains after the infection of a retrovirus carrying E7 oncogene of human papillomavirus type 16. MEB5 cells proliferated in serum-free, epidermal growth factor (EGF)-supplemented medium. They expressed markers for neural precursor cells (nestin, A2B5, and RC1) and did not express markers for neurons (class III beta-tubulin), astrocytes (glial fibrillary acidic protein), and oligodendrocytes (galactocerebroside). MEB5 cells were stably maintained in an undifferentiated state with a diploid karyotype in the presence of EGF. When they were deprived of EGF, about 50% of the cells died due to apoptosis within 24 h. The remaining cells differentiated into neurons, astrocytes, or oligodendrocytes within 2 wk. The newly developed cells with neuronal morphology were immunoreactive for gamma-aminobutyric acid and exhibited neuronal electrophysiological properties. When MEB5 cells were treated with leukemia inhibitory factor for 7 d, they were induced to differentiate exclusively into astrocytes. These results indicate that MEB5 is a cell line with characteristics of EGF-dependent, multipotent neural precursor cells. This cell line should provide a good model system to study the mechanisms of survival, proliferation, and differentiation of the multipotent precursor cells in the central nervous system. |
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Authors:
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Y Nakagaito; M Satoh; H Kuno; T Iwama; M Takeuchi; A Hakura; T Yoshida |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: In vitro cellular & developmental biology. Animal Volume: 34 ISSN: 1071-2690 ISO Abbreviation: In Vitro Cell. Dev. Biol. Anim. Publication Date: 1998 Jul-Aug |
Date Detail:
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Created Date: 1998-11-03 Completed Date: 1998-11-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9418515 Medline TA: In Vitro Cell Dev Biol Anim Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 585-92 Citation Subset: IM |
Affiliation:
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Institute for Fermentation, Osaka, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Astrocytes / cytology Cell Differentiation / physiology Cell Division / physiology Cell Line, Transformed Cell Survival / physiology Culture Media, Serum-Free Epidermal Growth Factor / physiology* Mice Mice, Inbred C3H Oncogene Proteins, Viral / genetics Papillomaviridae / genetics Phenotype Prosencephalon / cytology*, embryology, virology Retroviridae / genetics |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Serum-Free; 0/Oncogene Proteins, Viral; 0/oncogene protein E7, Human papillomavirus type 16; 62229-50-9/Epidermal Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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