| Establishment of an enzyme-linked immunosorbent assay with a coated deflagellated Salmonella enteritidis antigen for detection of a specific chicken antibody. | |
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MedLine Citation:
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PMID: 10879908 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We attempted to establish an enzyme-linked immunosorbent assay (ELISA) for field monitoring/profiling purposes for Salmonella enteritidis (SE) infection of poultry flocks. Serotyping rabbit sera, commercially obtained, specific for Salmonella identification sera to O2, O4, O7, O8, S. Vi, S. Hm, and O9, showed negative ELISA (E)-values (< 0.2) on ELISA, except the O9 identification serum (E-value > 0.5). Similar negative E-value results were obtained for antisera to Echerichia coli (E. O antigen). Field serum samples originating from SE-isolated flocks yielded similar positive ratios on both ELISAs including the present coated deflagellated SE antigen and a commercially obtained flagellated SE antigen and that of rapid plate aggregation with a pullorum antigen (PD-RPA). About 100 days after the first monitoring, no SE isolation in the same flock was observed resulting in a carrier state of SE infection. Although both the monitoring results with commercially obtained ELISA and PD-RPA showed lower positive or negative ratios, the present ELISA showed a higher positive ratio than that of the first monitoring. The present ELISA is suggested to be a suitable method to do accurate profiling on the carrier state of infection. |
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Authors:
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Y Yamane; N Awamura; H Fujii; H Ohta; Y Toyota; K Otsuki; T Inoue |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Avian diseases Volume: 44 ISSN: 0005-2086 ISO Abbreviation: Avian Dis. Publication Date: 2000 Apr-Jun |
Date Detail:
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Created Date: 2000-12-14 Completed Date: 2000-12-14 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370617 Medline TA: Avian Dis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 291-6 Citation Subset: IM |
Affiliation:
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CAF Laboratories, Hiroshima, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Bacterial / analysis* Carrier State / diagnosis, veterinary Chickens / immunology* Enzyme-Linked Immunosorbent Assay / methods, veterinary* Poultry Diseases / diagnosis Rabbits Salmonella Infections, Animal / diagnosis Salmonella enteritidis / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Bacterial |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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