| Establishment of a duck cell line susceptible to duck hepatitis virus type 1. | |
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MedLine Citation:
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PMID: 22633926 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Until recently, there was no cell line that could produce continuously high-titer duck hepatitis virus type 1 (DHV-1). In this study, a duck embryo fibroblast (DEF) cell line was established, and the susceptibility of this cell line to DHV-1 was determined. The primary culture of DEF cells was from a duck embryo that was partially digested with trypsin. Digested tissue pieces were cultured at 37°C in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum. The cultured DEF cells, which had the morphology of fibroblast, proliferated to 100% confluence four days later. An immortalized DEF cell line, named DEF-TA, was established and subcultured to passage 33, and the susceptibility of that cell line to DHV-1 was determined. In the DHV-1 susceptibility tests, cytopathic effects and the propagation of virus were observed in DEF-TA cells after DHV-1 infection. This continuous DHV-1-susceptible DEF cell line may serve as a valuable cell line for studies of cell-virus interactions and the pathogenesis of DHV-1 and may be useful for the development of an inactivated vaccine. |
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Authors:
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Yuzhi Fu; Zongyan Chen; Chuanfeng Li; Guangqing Liu |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-5-22 |
Journal Detail:
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Title: Journal of virological methods Volume: - ISSN: 1879-0984 ISO Abbreviation: - Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-5-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8005839 Medline TA: J Virol Methods Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Crown Copyright © 2012. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 200241, Shanghai, China; Animal Medical College, Hunan Agriculture University, Changsha, 410128, Changsha, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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