Document Detail


Establishment and cytogenetic characterization of a cell line from a pulmonary metastasis of osteosarcoma.
MedLine Citation:
PMID:  22833358     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Osteosarcoma (OS) is the most frequent malignant bone tumour in children and adolescents. In metastatic patients, the most common site of metastasis is the lung. There are relatively few cell lines of metastatic OS reported in the literature and the cytogenetic aspects of OS metastases are still controversial and inconclusive. Here we describe the establishment of a new OS cell line, M-OS, from a pulmonary metastasis of a typical osteoblastic OS of an 11-year-old boy with metastatic OS at diagnosis. M-OS cells have been maintained in culture for over 50 passages for more than 1 year. M-OS was characterized by immunohistochemistry, conventional cytogenetics and fluorescence in situ hybridization (FISH). In order to evaluate in vitro cell modification, the immunohistochemical analysis was performed in three different moments of the cell line: 10th, 30th and 50th passages. The conventional cytogenetic analysis revealed the ploidy of M-OS cell line as near-diploid, with most metaphases hyperdiploid and tetraploid. We found a copy number gain of MDM2 gene as the most frequent alteration in the FISH analysis. The immunohistochemical analysis confirmed that M-OS cell line maintained the osteogenic nature even after all passages for the cell line establishment in vitro.
Authors:
Carolina Salinas-Souza; Indhira Dias Oliveira; Renato de Oliveira; Maria Teresa de Seixas Alves; Antonio Sergio Petrilli; Silvia Regina Caminada Toledo
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Publication Detail:
Type:  Journal Article     Date:  2012-07-26
Journal Detail:
Title:  Cytotechnology     Volume:  65     ISSN:  0920-9069     ISO Abbreviation:  Cytotechnology     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-03-15     Completed Date:  2013-03-18     Revised Date:  2013-04-16    
Medline Journal Info:
Nlm Unique ID:  8807027     Medline TA:  Cytotechnology     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  347-53     Citation Subset:  -    
Affiliation:
Genetics Laboratory, Department of Pediatrics, Pediatric Oncology Institute (IOP/GRAACC/UNIFESP), Federal University of São Paulo, Rua Botucatu, 743-8° andar, São Paulo, SP, 04023-062, Brazil.
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