Document Detail


Establishment of conditionally immortalized mouse glomerular parietal epithelial cells in culture.
MedLine Citation:
PMID:  18596122     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Parietal epithelial cells (PEC) are major constituents of crescents in crescentic glomerulonephritis. The purpose of these studies was to establish an immortalized PEC cell line with similar characteristics to PEC in vivo for use in future mechanistic studies. Glomeruli were isolated from H-2Kb tsA58 transgenic mice (ImmortoMouse) by standard differential sieving, and several candidate PEC cell lines were obtained by subcloning outgrowths of cells from capsulated glomeruli. One clone, designated mouse PEC (mPEC), was extensively characterized. mPEC exhibited a compact cell body with typical epithelial morphology when grown in permissive conditions, but the cell shape changed to polygonal after 14 d in growth-restrictive conditions. mPEC but not podocytes used as a negative control expressed claudin-1, claudin-2, and protein gene product 9.5, which are proteins specific to PEC in vivo, and did not express the podocyte-specific proteins synaptopodin and nephrin. The junctional proteins zonula occludens-1 and beta-catenin stained positively in both mPEC and podocytes, but the staining pattern at cell-cell contacts was intermittent in mPEC and linear in podocytes. Finally, mPEC had thin bundled cortical F-actin filaments and no F-actin projections compared with podocytes, which exhibited thick bundled cortical F-actin filaments and interdigitating F-actin projections at cell-cell contacts. We conclude that immortalized mPEC in culture exhibit specific features of PEC in vivo and that these cells are distinct from podocytes, despite having the same mesenchymal origin. This mPEC line will assist in future mechanistic studies of PEC and enhance our understanding of glomerular injury.
Authors:
Takamoto Ohse; Jeffrey W Pippin; Michael R Vaughan; Paul T Brinkkoetter; Ronald D Krofft; Stuart J Shankland
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-07-02
Journal Detail:
Title:  Journal of the American Society of Nephrology : JASN     Volume:  19     ISSN:  1533-3450     ISO Abbreviation:  J. Am. Soc. Nephrol.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-29     Completed Date:  2008-10-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9013836     Medline TA:  J Am Soc Nephrol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1879-90     Citation Subset:  IM    
Affiliation:
Department of Medicine, Division of Nephrology, University of Washington, Seattle, WA 98195-6521, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques
Cell Differentiation
Cell Line*
Cell Survival
Clone Cells
Culture Media, Conditioned
Cytoskeletal Proteins / metabolism
Membrane Proteins / metabolism
Mice*
Mice, Transgenic
Podocytes / cytology*,  physiology*
Grant Support
ID/Acronym/Agency:
DK51096/DK/NIDDK NIH HHS; DK56799/DK/NIDDK NIH HHS; DK60525/DK/NIDDK NIH HHS; F32 DK070434/DK/NIDDK NIH HHS; K08DK076970/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Culture Media, Conditioned; 0/Cytoskeletal Proteins; 0/Membrane Proteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Successful treatment of class V+IV lupus nephritis with multitarget therapy.
Next Document:  KIBRA modulates directional migration of podocytes.