Document Detail

Establishment of clonal cell lines of taste buds from a p53(-/-) mouse tongue.
MedLine Citation:
PMID:  21437573     Owner:  NLM     Status:  MEDLINE    
A taste bud is a sensory organ and consists of 50-100 spindle-shaped cells. The cells function as taste acceptors. They have characteristics of both epithelial and neuronal cells. A taste bud contains four types of cells, type I, type II, type III cells, and basal cells. Taste buds were isolated from a tongue of a p53-deficient mouse at day 12, and 11 clonal taste bud (TBD) cell lines were established. In immunochemical analysis, all cell lines expressed cytokeratin 18, gustducin, T1R3, and neural cellular adhesion molecule, but not GLAST. In RT-PCR analysis, shh was not expressed in any of the cell lines. Further analysis with RT-PCR was conducted on four cell lines. They expressed G protein-coupled taste receptors; T1R3, T2R8 for sweet, bitter, umami. And they also expressed α-ENaC for salty taste. While, a candidate for sour receptor HCN4 was expressed in TBD-a1 and TBD-a7 lines. And another candidate for sour receptor PKD1L3 was slightly expressed in TBD-a1 and TBD-c1.
Hideyuki Sako; Makie Hori; Ikuo Masuho; Osamu Saitoh; Atsumasa Okada; Yasuhiro Tomooka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-03-25
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  47     ISSN:  1543-706X     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-04-26     Completed Date:  2011-08-22     Revised Date:  2011-10-27    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  333-40     Citation Subset:  IM    
Copyright Information:
© The Society for In Vitro Biology 2011
Department of Biological Science and Technology and Research Center for RNA Science, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
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MeSH Terms
Blotting, Western
Cell Line*
DNA Primers / genetics
Excitatory Amino Acid Transporter 1 / metabolism
Keratin-18 / metabolism
Mice, Knockout
Neural Cell Adhesion Molecules / metabolism
Receptors, G-Protein-Coupled / metabolism
Reverse Transcriptase Polymerase Chain Reaction
TRPP Cation Channels / metabolism
Taste Buds / cytology*
Tongue / cytology*
Transducin / metabolism
Tumor Suppressor Protein p53 / genetics*
Reg. No./Substance:
0/DNA Primers; 0/Excitatory Amino Acid Transporter 1; 0/Keratin-18; 0/Neural Cell Adhesion Molecules; 0/PKD1L3 protein, mouse; 0/Receptors, G-Protein-Coupled; 0/Slc1a3 protein, mouse; 0/TRPP Cation Channels; 0/Tumor Suppressor Protein p53; 147979-21-3/Gnat3 protein, rat; EC 3.6.1.-/Transducin
Comment In:
In Vitro Cell Dev Biol Anim. 2011 Sep;47(8):513-4; author reply 515   [PMID:  21720922 ]

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