Document Detail


Establishment of chimerism in donor liver with recipient-type bone marrow cells prior to liver transplantation produces marked suppression of allograft rejection in rats.
MedLine Citation:
PMID:  9664973     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, we investigated whether establishment of chimerism in donor liver with recipient-type bone marrow cells (BMCs) prior to liver transplantation could prolong the liver allograft survival. Donor female ACI rats were inoculated with recipient-type BMCs of male LEW rats via the portal vein, with or without irradiation as cytoablation, followed by intramuscular administration of FK506 for 5 days. At 1-2 months later, livers were harvested and transplanted into naive female LEW rats. No immunosuppressants were used. Chimerism in donor rats was confirmed by primers specific for the sex determinant Y chromosome of rats. With livers from rats pretreated with recipient-type BMCs, survival of liver allografts was significantly extended, irrespective of irradiation. These results showed that modification of the donor liver by intraportal injection of recipient-type BMCs and concomitant administration of FK506 prior to liver transplantation prolonged liver allograft survival in rats.
Authors:
O Sanada; Y Fukuda; R Sumimoto; S Hoshino; M Nishihara; K Kaneda; T Asahara; K Dohi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Transplant international : official journal of the European Society for Organ Transplantation     Volume:  11 Suppl 1     ISSN:  0934-0874     ISO Abbreviation:  Transpl. Int.     Publication Date:  1998  
Date Detail:
Created Date:  1998-11-30     Completed Date:  1998-11-30     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8908516     Medline TA:  Transpl Int     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  S174-8     Citation Subset:  IM    
Affiliation:
Second Department of Surgery, School of Medicine, Hiroshima University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Marrow Transplantation*
Dendritic Cells
Female
Graft Rejection / prevention & control*
Graft Survival
Liver Transplantation*
Male
Preoperative Care
Rats
Rats, Inbred ACI
Rats, Inbred Lew
Survival Rate
Transplantation Chimera*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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