| Establishment and characterization of two squamous cell carcinoma cell lines (HYVC and HMVC) derived from vulva. | |
MedLine Citation:
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PMID: 12889856 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Two cell lines, HYVC and HMVC, were established from cultures of the squamous cell carcinoma removed from the vulva of females of 37 and 46 years old, respectively. These cell lines were very similar in their biological characteristics, such as morphology (polygonal), growth pattern (32-43 hr of population doubling time, 50-25% of plating efficiency), heterotransplantability (1 x 10(7) cells produced squamous cell carcinomas in the nude mice), genetics (75-78 of modal chromosomal number), and producing the tumor markers (SCC and TPA). The HPV-DNA was not detected in either the HYVC or HMVC lines by using L1-PCR methods, however, it was detected in the HYVC using E6/E7-PCR. |
Authors:
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Kazushige Kiguchi; Isamu Ishiwata; Chieko Ishiwata; Masanori Iwata; Bunpei Ishizuka; Hiroyuki Yoshikawa; Toshiaki Tachibana; Hisashi Hashimoto; Hiroshi Ishikawa |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Human cell Volume: 15 ISSN: 0914-7470 ISO Abbreviation: Hum. Cell Publication Date: 2002 Dec |
Date Detail:
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Created Date: 2003-07-31 Completed Date: 2003-10-14 Revised Date: 2012-05-31 |
Medline Journal Info:
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Nlm Unique ID: 8912329 Medline TA: Hum Cell Country: Japan |
Other Details:
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Languages: eng Pagination: 207-14 Citation Subset: IM |
Affiliation:
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Dept. Ob. & Gy., Toyoko Hosp., Sch. Of Med. St. Marianna Univ. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Animals Carcinoembryonic Antigen / metabolism Carcinoma, Squamous Cell / genetics, metabolism, pathology*, virology Cell Division Female Humans Karyotyping Mice Mice, Nude Middle Aged Papillomaviridae / isolation & purification Transplantation, Heterologous Tumor Cells, Cultured* Vulvar Neoplasms / genetics, metabolism, pathology*, virology |
| Chemical | |
Reg. No./Substance:
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0/Carcinoembryonic Antigen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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