Document Detail


Establishment and characterization of two squamous cell carcinoma cell lines (HYVC and HMVC) derived from vulva.
MedLine Citation:
PMID:  12889856     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two cell lines, HYVC and HMVC, were established from cultures of the squamous cell carcinoma removed from the vulva of females of 37 and 46 years old, respectively. These cell lines were very similar in their biological characteristics, such as morphology (polygonal), growth pattern (32-43 hr of population doubling time, 50-25% of plating efficiency), heterotransplantability (1 x 10(7) cells produced squamous cell carcinomas in the nude mice), genetics (75-78 of modal chromosomal number), and producing the tumor markers (SCC and TPA). The HPV-DNA was not detected in either the HYVC or HMVC lines by using L1-PCR methods, however, it was detected in the HYVC using E6/E7-PCR.
Authors:
Kazushige Kiguchi; Isamu Ishiwata; Chieko Ishiwata; Masanori Iwata; Bunpei Ishizuka; Hiroyuki Yoshikawa; Toshiaki Tachibana; Hisashi Hashimoto; Hiroshi Ishikawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human cell     Volume:  15     ISSN:  0914-7470     ISO Abbreviation:  Hum. Cell     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2003-07-31     Completed Date:  2003-10-14     Revised Date:  2012-05-31    
Medline Journal Info:
Nlm Unique ID:  8912329     Medline TA:  Hum Cell     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  207-14     Citation Subset:  IM    
Affiliation:
Dept. Ob. & Gy., Toyoko Hosp., Sch. Of Med. St. Marianna Univ.
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MeSH Terms
Descriptor/Qualifier:
Adult
Animals
Carcinoembryonic Antigen / metabolism
Carcinoma, Squamous Cell / genetics,  metabolism,  pathology*,  virology
Cell Division
Female
Humans
Karyotyping
Mice
Mice, Nude
Middle Aged
Papillomaviridae / isolation & purification
Transplantation, Heterologous
Tumor Cells, Cultured*
Vulvar Neoplasms / genetics,  metabolism,  pathology*,  virology
Chemical
Reg. No./Substance:
0/Carcinoembryonic Antigen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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