| Establishment and characterization of a steroidogenic human granulosa-like tumor cell line, KGN, that expresses functional follicle-stimulating hormone receptor. | |
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MedLine Citation:
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PMID: 11145608 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We established a steroidogenic human ovarian granulosa-like tumor cell line, designated KGN, from a patient with invasive ovarian granulosa cell carcinoma. KGN had a relatively long population doubling time of about 46.4 h and had an abnormal karyotype of 45,XX, 7q-, -22. A steroid analysis of the cultured medium by RIA performed 5 yr after the initiation of culture showed that KGN was able to secrete pregnenolone and progesterone, and both dramatically increased after stimulation with (Bu)(2)cAMP. However, little or no secretion of 17alpha-hydroxylated steroids, dehydroepiandrosterone, androstenedione, or estradiol was observed. The aromatase activity of KGN was relatively high and was further stimulated by (Bu)(2)cAMP or FSH. These findings showed a pattern similar to that of steroidogenesis in human granulosa cells, thus allowing analysis of naturally occurring steroidogenesis in human granulosa cells. Fas-mediated apoptosis of KGN was also observed, which mimicked the physiological regulation of apoptosis in normal human granulosa cells. Based on these findings, this cell line is considered to be a very useful model for understanding the regulation of steroidogenesis, cell growth, and apoptosis in human granulosa cells. |
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Authors:
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Y Nishi; T Yanase; Y Mu; K Oba; I Ichino; M Saito; M Nomura; C Mukasa; T Okabe; K Goto; R Takayanagi; Y Kashimura; M Haji; H Nawata |
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Publication Detail:
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Type: Case Reports; Journal Article |
Journal Detail:
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Title: Endocrinology Volume: 142 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2001-01-26 Completed Date: 2001-03-01 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
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Languages: eng Pagination: 437-45 Citation Subset: AIM; IM |
Affiliation:
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Department of Medicine and Bioregulatory Science (Third Department of Internal Medicine), Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka 812-8582, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects Aromatase / metabolism Bucladesine / pharmacology Cell Culture Techniques / methods Cell Division Chromosome Aberrations Cyclic AMP / metabolism Female Follicle Stimulating Hormone / metabolism Granulosa Cell Tumor / genetics, pathology*, physiopathology Humans Interferon-gamma, Recombinant / pharmacology Karyotyping Middle Aged Ovarian Neoplasms / genetics, pathology*, physiopathology Pregnenolone / secretion Progesterone / metabolism, secretion Receptors, FSH / metabolism* Tetradecanoylphorbol Acetate / pharmacology Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Interferon-gamma, Recombinant; 0/Receptors, FSH; 145-13-1/Pregnenolone; 16561-29-8/Tetradecanoylphorbol Acetate; 362-74-3/Bucladesine; 57-83-0/Progesterone; 60-92-4/Cyclic AMP; 9002-68-0/Follicle Stimulating Hormone; EC 1.14.14.1/Aromatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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