Document Detail


Establishment and characterization of a spindle cell squamous carcinoma cell line.
MedLine Citation:
PMID:  16918599     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Spindle cell squamous carcinoma (SCSC) is a rare and peculiar biphasic malignant neoplasm that occurs mainly in the upper aerodigestive tract. It consists of sarcomatoid proliferation of pleomorphic spindle-shaped cells and squamous cell carcinoma. METHODS: Here, we established a SCSC cell line from a tumour arisen in gingiva. We characterized the feature of a SCSC cell line by immunohistochemistry. To know the biological feature, we examined the cell growth, invasiveness and epithelial-mesenchymal transition markers of a SCSC cell line in comparison with oral squamous cell carcinoma (OSCC) cell lines. RESULTS: By immunohistochemical analyses, the primary tumour expressed cytokeratin and vimentin, indicating carcinosarcoma-like characters. This tumour also showed overexpression of p53 protein. Cultured SCSC cells resulted in bypass of crisis and maintenance over passage 100. The established SCSC cell line was spindle-shaped and showed identical immunohistochemical characters to those of primary tumour cells. Similar to the primary tumour, the cell line showed p53 overexpression and had p53 mutation at codon 132: AAG (lys)-->AAT (asp). The SCSC cell line grew slower than two other OSCC cell lines (MSCC-1 and HSC-2), whereas SCSC cells had remarkable invasiveness in comparison with these cell lines. Moreover, SCSC cells expressed wnt-5a and vimentin mRNA at high levels, but did not express E-cadherin mRNA. This expression pattern of the markers was similar to that of mesenchymal cells, not of epithelial cells. CONCLUSION: In the present study, we newly established a SCSC cell line with strong invasiveness. This is the first report on the establishment of SCSC cell line. The SCSC cell line can be a useful cell model for the study to know the cytodifferentiation and nature of SCSC.
Authors:
Yasusei Kudo; Ikuko Ogawa; Masae Kitagawa; Shojiro Kitajima; B S M Samadarani Siriwardena; Noriko Aobara; Chika Matsuda; Mutsumi Miyauchi; Takashi Takata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology     Volume:  35     ISSN:  0904-2512     ISO Abbreviation:  J. Oral Pathol. Med.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-08-21     Completed Date:  2006-11-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8911934     Medline TA:  J Oral Pathol Med     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  479-83     Citation Subset:  D; IM    
Affiliation:
Department of Oral Maxillofacial Pathobiology, Division of Frontier Medical Science, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan. ykudo@hiroshima-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Aged
Cadherins / analysis
Carcinoma, Squamous Cell* / chemistry,  pathology
Cell Line, Tumor* / chemistry,  pathology
Gingival Neoplasms* / chemistry,  pathology
Humans
Keratins / isolation & purification
Male
Peptide Fragments / isolation & purification
Tumor Markers, Biological / analysis
Tumor Suppressor Protein p53 / isolation & purification
Vimentin / isolation & purification
Chemical
Reg. No./Substance:
0/Cadherins; 0/Peptide Fragments; 0/Tumor Markers, Biological; 0/Tumor Suppressor Protein p53; 0/Vimentin; 0/p53 protein (325-355), human; 68238-35-7/Keratins

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