Document Detail

Establishment and characterization of an osteopontin-null cutaneous squamous cell carcinoma cell line.
MedLine Citation:
PMID:  19915934     Owner:  NLM     Status:  MEDLINE    
Osteopontin (OPN) is a secreted glycoprotein implicated to function in cancer development and metastasis. Although elevated expression of OPN are observed in cancer cells of various types, in some cases, only the cells in the stromal region surrounding the tumor express OPN, suggesting distinct functional roles for this protein derived from host cells and from cancer cells. To provide a model for addressing the functions and mechanisms of host-derived OPN in cancer progression and metastasis, a cutaneous squamous cell carcinoma cell line (ONSC) that lacks the OPN gene, Spp1, was established. This line of cells was derived from a squamous cell carcinoma that developed in a female, OPN-null mouse subjected to two-stage skin carcinogenesis. Morphologically, ONSC cells resemble epithelial cells, and they express the epithelial markers, K1, K14, and p63, as confirmed by immunohistochemical analyses. Genomic analyses indicate the presence of mutated H-Ras and p53 genes. ONSC cells form colonies in soft agar and, subcutaneously injected into athymic nude mice, develop into squamous cell carcinomas that metastasize to the lungs. Lacking OPN expression, these squamous cell carcinoma cells provide a model to address the function of host OPN in the context of cancer progression and metastasis.
Yu-Hua Hsieh; M Margaret Juliana; Pi-Ling Chang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  In vitro cellular & developmental biology. Animal     Volume:  46     ISSN:  1543-706X     ISO Abbreviation:  In Vitro Cell. Dev. Biol. Anim.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2011-04-11     Completed Date:  2011-07-26     Revised Date:  2013-05-31    
Medline Journal Info:
Nlm Unique ID:  9418515     Medline TA:  In Vitro Cell Dev Biol Anim     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  87-91     Citation Subset:  IM    
Department of Nutrition Sciences, University of Alabama at Birmingham, 1530 3rd Avenue South, 311 Webb, Birmingham, AL 35294, USA.
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MeSH Terms
Carcinoma, Squamous Cell / genetics,  pathology,  secondary*
Cell Line, Tumor*
Gene Deletion
Genetic Markers
Lung Neoplasms / secondary
Mice, Nude
Neoplasm Metastasis / genetics,  pathology*
Osteopontin / genetics*,  physiology
Skin Neoplasms / genetics,  pathology*
Grant Support
R01 CA090920/CA/NCI NIH HHS; R01 CA137091/CA/NCI NIH HHS; R01 CA90920/CA/NCI NIH HHS
Reg. No./Substance:
0/Genetic Markers; 0/Spp1 protein, mouse; 106441-73-0/Osteopontin

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