Document Detail


Establishment and characterization of a new leukaemic T-cell line (Peer) with an unusual phenotype.
MedLine Citation:
PMID:  14768698     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report the isolation and establishment in continuous culture of a human lymphoid cell line (Peer) from a case of T-leukemia. The Peer cell line lacks some typical cell-surface properties of T cells, namely sheep erythrocyte rosette formation and reactivity with two anti-T-cell sera, but has focal acid phosphatase and does express two other T-cell antigens, one defined by a monoclonal antibody, the other related to a T-cell subset (TH2). The cells are negative for B-cell markers (SmIg or cytoplasmic mu Fcgamma and C3 receptors, mouse erythrocyte rosettes) and EBV (EBNA). In addition, the Peer cell does not possess the typical phenotypic markers of "non-B, non-T" leukemia: cALL and Ia-like antigens, and the cytoplasmic hexosaminidase isoenzyme I, but is positive for terminal deoxynucleotidyl transferase by enzymatic and immunofluorescent criteria. The cell line requires exogenous L-asparagine for adequate growth in culture, a property known to be characteristic of certain T cells but not of B cells. The Peer cell line appears to have a maturation arrest at a developmental stage intermediate between the cortical thymocyte and a mature T-cell subset and to have lost some T-cell differentiation features.
Authors:
Z Ravid; N Goldblum; R Zaizov; M Schlesinger; T Kertes; J Minowada; W Verbi; M Greaves
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Publication Detail:
Type:  Case Reports; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  25     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1980 Jun 
Date Detail:
Created Date:  2004-02-10     Completed Date:  2004-02-24     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  705-10     Citation Subset:  IM    
Affiliation:
Chanock Centre for Virology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Neoplasm / blood
Antineoplastic Combined Chemotherapy Protocols / therapeutic use
Cell Culture Techniques / methods
Cell Nucleus / pathology
Child, Preschool
Fatal Outcome
Female
Humans
Phenotype
Precursor Cell Lymphoblastic Leukemia-Lymphoma / drug therapy,  immunology,  pathology*
Rosette Formation
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Antigens, Neoplasm

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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