| Establishment and characterization of a new human gallbladder carcinoma cell line. | |
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MedLine Citation:
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PMID: 22843894 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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BACKGROUND: Prognosis for patients with gallbladder carcinoma (GBC) is poor and the standard treatment for GBC has not yet been established. MATERIALS AND METHODS: We established the human GBC cell line TYGBK-1, from a patient with papillary, tubular adenocarcinoma. RESULTS: The doubling time was 48 hours. This cell line has a missense mutation of p53 and no mutation of the K-RAS gene. This cell line was transplantable to nude mice. We characterized the sensitivity of TYGBK-1 to gemcitabine. We also examined the association of two gemcitabine-related genes (deoxycytidine kinase, dCK, and Hu antigen R, HuR). Among four GBC cell lines (TYGBK-1, NOZ, G-415, TGBC2TKB), TYGBK-1 and NOZ exhibited sensitivity to gemcitabine. Furthermore, these cells expressed both dCK and HuR mRNA, rather than gemcitabine-resistant cells. CONCLUSION: The newly established GBC cell line TYGBK-1, may represent an effective tool for development of chemotherapeutic treatment for GBC. |
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Authors:
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Shinichi Sekine; Yutaka Shimada; Takuya Nagata; Makoto Moriyama; Testuya Omura; Isaku Yoshioka; Ryouta Hori; Koushi Matsui; Shigeaki Sawada; Tomoyuki Okumura; Toru Yoshida; Kazuhiro Tsukada |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Anticancer research Volume: 32 ISSN: 1791-7530 ISO Abbreviation: Anticancer Res. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-07-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: Greece |
Other Details:
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Languages: eng Pagination: 3211-8 Citation Subset: IM |
Affiliation:
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Department of Surgery and Science, University of Toyama, 2630 Sugitani, Toyama-city, Toyama, 930-0194, Japan. sekky@med.u-toyama.ac.jp. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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