| Establishment and characterization of a new highly metastatic human osteosarcoma cell line. | |
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MedLine Citation:
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PMID: 19363654 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Osteosarcoma is the most common primary malignancy of bone in children and young adults. There is a paucity of tumorigenic and highly metastatic human osteosarcoma cell lines that have not been further transformed by exogenous means. Here we establish and characterize a highly metastatic human osteosarcoma cell line that is derived from a poorly metastatic MG63 line through serial passage in nude mice via intratibial injections. The occasional pulmonary metastases developed from MG63 were harvested and repassaged in mice until a highly metastatic subline (MG63.2) was established. The parental MG63 and highly metastatic MG63.2 cells were further characterized in vitro and in vivo. MG63.2 cells demonstrated increased cell migration and invasion compared to the parental MG63 cells. Conversely, cell adhesion was significantly greater in MG63 cells when compared to the MG63.2 cells. MG63.2 cells grew at a slightly slower rate than that of the parental cells. When injected into nude mice, MG63.2 cells had a greater than 200-fold increase in developing pulmonary metastases compared to the parental MG63 cells. MG63.2 cells also formed larger primary tumors when compared to the parental MG63 cells. Further analysis revealed that ezrin expression was up-regulated in the metastatic MG63.2 cells. Interestingly, expressions of MMP-2 and MMP-9 were down-regulated, and expression of TIMP-2 was up-regulated in the MG63.2 cells. Taken together, we have established and characterized a highly metastatic human osteosarcoma cell line that should serve as a valuable tool for future investigations on the pathogenesis, metastasis, and potential treatments of human osteosarcoma. |
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Authors:
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Yuxi Su; Xiaoji Luo; Bai-Cheng He; Yi Wang; Liang Chen; Guo-Wei Zuo; Bo Liu; Yang Bi; Jiayi Huang; Gao-Hui Zhu; Yun He; Quan Kang; Jinyong Luo; Jikun Shen; Jin Chen; Xianqing Jin; Rex C Haydon; Tong-Chuan He; Hue H Luu |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2009-04-11 |
Journal Detail:
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Title: Clinical & experimental metastasis Volume: 26 ISSN: 1573-7276 ISO Abbreviation: Clin. Exp. Metastasis Publication Date: 2009 |
Date Detail:
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Created Date: 2009-11-12 Completed Date: 2009-11-27 Revised Date: 2010-07-08 |
Medline Journal Info:
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Nlm Unique ID: 8409970 Medline TA: Clin Exp Metastasis Country: Netherlands |
Other Details:
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Languages: eng Pagination: 599-610 Citation Subset: IM |
Affiliation:
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The Children's Hospital and Key Laboratory of Diagnostic Medicine Designated by the Ministry of Education of China, Chongqing Medical University, 400016, Chongqing, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Blotting, Western Bone Neoplasms / genetics, pathology* Cell Adhesion Cell Line, Tumor DNA Primers Gene Expression Regulation, Neoplastic Humans Male Mice Mice, Nude Neoplasm Invasiveness Neoplasm Metastasis* Osteoblastoma / genetics, pathology* Reverse Transcriptase Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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