Document Detail


Establishment and characterization of a new highly metastatic human osteosarcoma cell line.
MedLine Citation:
PMID:  19363654     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Osteosarcoma is the most common primary malignancy of bone in children and young adults. There is a paucity of tumorigenic and highly metastatic human osteosarcoma cell lines that have not been further transformed by exogenous means. Here we establish and characterize a highly metastatic human osteosarcoma cell line that is derived from a poorly metastatic MG63 line through serial passage in nude mice via intratibial injections. The occasional pulmonary metastases developed from MG63 were harvested and repassaged in mice until a highly metastatic subline (MG63.2) was established. The parental MG63 and highly metastatic MG63.2 cells were further characterized in vitro and in vivo. MG63.2 cells demonstrated increased cell migration and invasion compared to the parental MG63 cells. Conversely, cell adhesion was significantly greater in MG63 cells when compared to the MG63.2 cells. MG63.2 cells grew at a slightly slower rate than that of the parental cells. When injected into nude mice, MG63.2 cells had a greater than 200-fold increase in developing pulmonary metastases compared to the parental MG63 cells. MG63.2 cells also formed larger primary tumors when compared to the parental MG63 cells. Further analysis revealed that ezrin expression was up-regulated in the metastatic MG63.2 cells. Interestingly, expressions of MMP-2 and MMP-9 were down-regulated, and expression of TIMP-2 was up-regulated in the MG63.2 cells. Taken together, we have established and characterized a highly metastatic human osteosarcoma cell line that should serve as a valuable tool for future investigations on the pathogenesis, metastasis, and potential treatments of human osteosarcoma.
Authors:
Yuxi Su; Xiaoji Luo; Bai-Cheng He; Yi Wang; Liang Chen; Guo-Wei Zuo; Bo Liu; Yang Bi; Jiayi Huang; Gao-Hui Zhu; Yun He; Quan Kang; Jinyong Luo; Jikun Shen; Jin Chen; Xianqing Jin; Rex C Haydon; Tong-Chuan He; Hue H Luu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2009-04-11
Journal Detail:
Title:  Clinical & experimental metastasis     Volume:  26     ISSN:  1573-7276     ISO Abbreviation:  Clin. Exp. Metastasis     Publication Date:  2009  
Date Detail:
Created Date:  2009-11-12     Completed Date:  2009-11-27     Revised Date:  2010-07-08    
Medline Journal Info:
Nlm Unique ID:  8409970     Medline TA:  Clin Exp Metastasis     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  599-610     Citation Subset:  IM    
Affiliation:
The Children's Hospital and Key Laboratory of Diagnostic Medicine Designated by the Ministry of Education of China, Chongqing Medical University, 400016, Chongqing, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Blotting, Western
Bone Neoplasms / genetics,  pathology*
Cell Adhesion
Cell Line, Tumor
DNA Primers
Gene Expression Regulation, Neoplastic
Humans
Male
Mice
Mice, Nude
Neoplasm Invasiveness
Neoplasm Metastasis*
Osteoblastoma / genetics,  pathology*
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/DNA Primers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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