Document Detail


Establishment and characterization of multidrug-resistant gastric cancer cell lines.
MedLine Citation:
PMID:  20393015     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
MATERIALS AND METHODS: Five cancer cell lines resistant to 5-fluorouracil, paclitaxel, oxaliplatin, irinotecan, or gemcitabine, were respectively established from a parent gastric cancer cell line, OCUM-2M, by stepwise exposure to each chemotherapeutical agent.
RESULTS: Cell death by apoptosis induced by anti-cancer drugs was low in 5 chemo-resistant cell lines. Percentage of cells in S and G(0)/G(1) phase was low in cell lines resistant to oxaliplatin or irinotecan. Cell lines resistant to paclitaxel, oxaliplatin, and gemcitabine showed multi-drug resistance. Alterations in MRP, DAPK1, or DAPK2 expression were found in multi-drug resistant cell lines.
CONCLUSION: The cell-cycle distribution and alterations of MRP, DAPK1, and DAPK2 genes may be integral part of mechanisms responsible for chemo-resistance. These cell lines might be useful to study molecular mechanisms leading to multi-drug resistance.
Authors:
Xiaotian Zhang; Masakazu Yashiro; Hong Qiu; Takafumi Nishii; Taro Matsuzaki; Kosei Hirakawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-04-15     Completed Date:  2010-05-18     Revised Date:  2013-06-03    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  915-21     Citation Subset:  IM    
Affiliation:
Department of Surgical Oncology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, Osaka 545-8585, Japan.
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents, Phytogenic / pharmacology
Apoptosis / drug effects,  physiology
Apoptosis Regulatory Proteins / biosynthesis
Calcium-Calmodulin-Dependent Protein Kinases / biosynthesis
Camptothecin / analogs & derivatives,  pharmacology
Cell Cycle / drug effects,  physiology
Cell Line, Tumor*
Deoxycytidine / analogs & derivatives,  pharmacology
Drug Resistance, Multiple
Drug Resistance, Neoplasm
Humans
Organoplatinum Compounds / pharmacology
P-Glycoproteins / biosynthesis
Paclitaxel / pharmacology
Reverse Transcriptase Polymerase Chain Reaction
Stomach Neoplasms / drug therapy*,  genetics,  metabolism,  pathology*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Apoptosis Regulatory Proteins; 0/Organoplatinum Compounds; 0/P-Glycoproteins; 33069-62-4/Paclitaxel; 63121-00-6/oxaliplatin; 7673326042/irinotecan; 7689-03-4/Camptothecin; 951-77-9/Deoxycytidine; B76N6SBZ8R/gemcitabine; EC 2.7.11.1/death-associated protein kinase; EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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