Document Detail

Establishment and characterization of multidrug-resistant gastric cancer cell lines.
MedLine Citation:
PMID:  20393015     Owner:  NLM     Status:  MEDLINE    
AIM: The aim of this study was to establish drug-resistant cell lines and to elucidate mechanisms leading to multi-drug resistance in gastric cancer.
MATERIALS AND METHODS: Five cancer cell lines resistant to 5-fluorouracil, paclitaxel, oxaliplatin, irinotecan, or gemcitabine, were respectively established from a parent gastric cancer cell line, OCUM-2M, by stepwise exposure to each chemotherapeutical agent.
RESULTS: Cell death by apoptosis induced by anti-cancer drugs was low in 5 chemo-resistant cell lines. Percentage of cells in S and G(0)/G(1) phase was low in cell lines resistant to oxaliplatin or irinotecan. Cell lines resistant to paclitaxel, oxaliplatin, and gemcitabine showed multi-drug resistance. Alterations in MRP, DAPK1, or DAPK2 expression were found in multi-drug resistant cell lines.
CONCLUSION: The cell-cycle distribution and alterations of MRP, DAPK1, and DAPK2 genes may be integral part of mechanisms responsible for chemo-resistance. These cell lines might be useful to study molecular mechanisms leading to multi-drug resistance.
Xiaotian Zhang; Masakazu Yashiro; Hong Qiu; Takafumi Nishii; Taro Matsuzaki; Kosei Hirakawa
Related Documents :
14610615 - Activity of docetaxel in paclitaxel-resistant ovarian cancer cells.
1348015 - Biochemical characterization of resistance to mitoxantrone and adriamycin in caco-2 hum...
8705215 - Determination and reversal of resistance to epirubicin intravesical chemotherapy. a con...
1645555 - Study of molecular markers of resistance to m-amsa in a human breast cancer cell line. ...
20347135 - Exploiting the superior protein resistance of polymer brushes to control single cell ad...
2653105 - An immunohistochemical study on the embryonic development of renin-containing cells in ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Anticancer research     Volume:  30     ISSN:  1791-7530     ISO Abbreviation:  Anticancer Res.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-04-15     Completed Date:  2010-05-18     Revised Date:  2014-07-30    
Medline Journal Info:
Nlm Unique ID:  8102988     Medline TA:  Anticancer Res     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  915-21     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antineoplastic Agents, Phytogenic / pharmacology
Apoptosis / drug effects,  physiology
Apoptosis Regulatory Proteins / biosynthesis
Calcium-Calmodulin-Dependent Protein Kinases / biosynthesis
Camptothecin / analogs & derivatives,  pharmacology
Cell Cycle / drug effects,  physiology
Cell Line, Tumor*
Death-Associated Protein Kinases
Deoxycytidine / analogs & derivatives,  pharmacology
Drug Resistance, Multiple
Drug Resistance, Neoplasm
Organoplatinum Compounds / pharmacology
P-Glycoproteins / biosynthesis
Paclitaxel / pharmacology
Reverse Transcriptase Polymerase Chain Reaction
Stomach Neoplasms / drug therapy*,  genetics,  metabolism,  pathology*
Reg. No./Substance:
0/Antineoplastic Agents, Phytogenic; 0/Apoptosis Regulatory Proteins; 0/Organoplatinum Compounds; 0/P-Glycoproteins; 0W860991D6/Deoxycytidine; 33069-62-4/Paclitaxel; 63121-00-6/oxaliplatin; 7673326042/irinotecan; B76N6SBZ8R/gemcitabine; EC protein, human; EC protein, human; EC Protein Kinases; EC Protein Kinases; XT3Z54Z28A/Camptothecin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Effects of insulin-like growth factor binding protein 7 on apoptosis in human teratocarcinoma cells ...
Next Document:  3-Bromopyruvate induces endoplasmic reticulum stress, overcomes autophagy and causes apoptosis in hu...