| Establishment and characterization of a lactating bovine mammary epithelial cell model for the study of milk synthesis. | |
| | |
MedLine Citation:
|
PMID: 20214659 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
This study sought to establish an in vitro lactating BMEC (bovine mammary epithelial cell) model, which may maintain the native function for a period of time. Mammary tissues of midlactation Holstein dairy cows were dispersed and cultured in a medium containing insulin, prolactin, hydrocortisone, transferrin, epidermal growth factor and fetal calf serum. After the cells migrating from the tissue reached approximately 80% of confluency, the tissues were removed, and secretory epithelial cells were enriched by digesting with 0.25% trypsin repeatedly to remove fibroblasts. The BMEC cells plated on plastic dishes displayed a monolayer, cobblestone, epithelial-like morphology and formed alveoli-like structures and island monolayer aggregates which are the typical characteristics of the mammary epithelial cells. The isolated cells were identified as of epithelial origin by staining with antibody against cytokeratin 18. A one-half logarithmically growth curve and abundant microvilli and cytoplasmic lipid droplets were observed in these cells. The transcription of the alphas1 casein gene and synthesis of alphas caseins were also detected in the model. Thus, our lactating BMEC model can be an effective model in vitro for studies of milk synthesis in the bovine mammary gland. |
| | |
Authors:
|
Ke Zhao; Hong-Yun Liu; Miao-Miao Zhou; Jian-Xin Liu |
Related Documents
:
|
2580429 - Paget's cells. new evidence linking mammary and extramammary paget cells to a common ce... 10927049 - Early-stage endometriosis: adhesion and growth of human menstrual endometrium in nude m... 19862839 - Lineage enforcement by inductive mesenchyme on adult epithelial stem cells across devel... 7215309 - Estrogen action in the mouse uterus: differential nuclear localization of estradiol in ... 11162929 - Characterization of androgen receptors in a well-differentiated endometrial adenocarcin... 15998499 - Ovarian and igf-i axis control of mammary development in prepubertal heifers. 16003719 - Assay to measure cd59 mutations in cho a(l) cells using flow cytometry. 23182339 - Taurine attenuates apoptosis in primary liver cells isolated from atlantic salmon (salm... 18486629 - Keratin-protein kinase c interaction in reactive oxygen species-induced hepatic cell de... |
Publication Detail:
|
Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Cell biology international Volume: 34 ISSN: 1095-8355 ISO Abbreviation: Cell Biol. Int. Publication Date: 2010 Jul |
Date Detail:
|
Created Date: 2010-06-10 Completed Date: 2010-09-23 Revised Date: 2010-11-12 |
Medline Journal Info:
|
Nlm Unique ID: 9307129 Medline TA: Cell Biol Int Country: England |
Other Details:
|
Languages: eng Pagination: 717-21 Citation Subset: IM |
Affiliation:
|
Institute of Dairy Science, MOE Key Laboratory of Molecular Animal Nutrition, Zhejiang University, No. 268 Kaixuan Road, Hangzhou 310029, Peoples Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Caseins / genetics, metabolism Cattle Cell Shape Cells, Cultured Epithelial Cells* / physiology, ultrastructure Female Keratin-18 / metabolism Lactation / physiology* Mammary Glands, Animal* / cytology, physiology Milk / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Caseins; 0/Keratin-18 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evidence for possible biological advantages of the newly emerging HIV-1 circulating recombinant form...
Next Document: Antigen dose-dependent suppression of murine IgE responses is mediated by CD4CD8 double-negative T c...