Document Detail


Establishment and characterization of a lactating bovine mammary epithelial cell model for the study of milk synthesis.
MedLine Citation:
PMID:  20214659     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study sought to establish an in vitro lactating BMEC (bovine mammary epithelial cell) model, which may maintain the native function for a period of time. Mammary tissues of midlactation Holstein dairy cows were dispersed and cultured in a medium containing insulin, prolactin, hydrocortisone, transferrin, epidermal growth factor and fetal calf serum. After the cells migrating from the tissue reached approximately 80% of confluency, the tissues were removed, and secretory epithelial cells were enriched by digesting with 0.25% trypsin repeatedly to remove fibroblasts. The BMEC cells plated on plastic dishes displayed a monolayer, cobblestone, epithelial-like morphology and formed alveoli-like structures and island monolayer aggregates which are the typical characteristics of the mammary epithelial cells. The isolated cells were identified as of epithelial origin by staining with antibody against cytokeratin 18. A one-half logarithmically growth curve and abundant microvilli and cytoplasmic lipid droplets were observed in these cells. The transcription of the alphas1 casein gene and synthesis of alphas caseins were also detected in the model. Thus, our lactating BMEC model can be an effective model in vitro for studies of milk synthesis in the bovine mammary gland.
Authors:
Ke Zhao; Hong-Yun Liu; Miao-Miao Zhou; Jian-Xin Liu
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  34     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-10     Completed Date:  2010-09-23     Revised Date:  2010-11-12    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  717-21     Citation Subset:  IM    
Affiliation:
Institute of Dairy Science, MOE Key Laboratory of Molecular Animal Nutrition, Zhejiang University, No. 268 Kaixuan Road, Hangzhou 310029, Peoples Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Caseins / genetics,  metabolism
Cattle
Cell Shape
Cells, Cultured
Epithelial Cells* / physiology,  ultrastructure
Female
Keratin-18 / metabolism
Lactation / physiology*
Mammary Glands, Animal* / cytology,  physiology
Milk / metabolism*
Chemical
Reg. No./Substance:
0/Caseins; 0/Keratin-18

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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