| Establishment and characterization of an immortalized Z310 choroidal epithelial cell line from murine choroid plexus. | |
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MedLine Citation:
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PMID: 12470873 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The choroid plexus plays a wide range of roles in brain development, maturation, aging process, endocrine regulation, and pathogenesis of certain neurodegenerative diseases. To facilitate in vitro study, we have used a gene transfection technique to immortalize murine choroidal epithelial cells. A viral plasmid (pSV3neo) was inserted into the host genome of primary choroidal epithelia by calcium phosphate precipitation. The transfected epithelial cells, i.e., Z310 cells, that survived from cytotoxic selection expressed SV40 large-T antigen throughout the life span, suggesting a successful gene transfection. The cells displayed the same polygonal epithelial morphology as the starting cells by light microscopy. Immunocytochemical studies demonstrate the presence of transthyretin (TTR), a thyroxine transport protein known to be exclusively produced by the choroidal epithelia in the CNS, in both transfected and starting cells. Western blot analyses further confirm the production and secretion of TTR by these cells. The mRNAs encoding transferrin receptor (TfR) were identified by Northern blot analyses. The cells grow at a steady rate, currently in the 110th passage with a population doubling time of 20-22 h in the established culture. When Z310 cells were cultured onto a Trans-well apparatus, the cells formed an epithelial monolayer similar to primary choroidal cells, possessing features such as an uneven fluid level between inner and outer chambers and an electrical resistance approximately 150-200 omega-cm(2). These results indicate that immortalized Z310 cells possess the characteristics of choroidal epithelia and may have the potential for application in blood-CSF barrier (BCB) research. |
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Authors:
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Wei Zheng; Qiuqu Zhao |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Brain research Volume: 958 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 2002 Dec |
Date Detail:
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Created Date: 2002-12-09 Completed Date: 2003-03-27 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 371-80 Citation Subset: IM |
Copyright Information:
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Copyright 2002 Elsevier Science B.V. |
Affiliation:
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Department of Environmental Health Sciences, Columbia University School of Public Health, 60 Haven Ave, B1-110, New York, NY 10032, USA. wz18@columbia.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Polyomavirus Transforming / genetics Cell Culture Techniques / methods* Cell Line, Transformed / cytology*, virology Cell Transformation, Viral / physiology Choroid Plexus / chemistry, cytology* Epithelial Cells / chemistry, cytology*, virology Female Male Plasmids / genetics Rats Rats, Sprague-Dawley Transfection / methods |
| Grant Support | |
ID/Acronym/Agency:
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ES08146/ES/NIEHS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Polyomavirus Transforming |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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