Document Detail


Establishment and characterization of a human-papillomavirus negative, p53-mutation negative human cervical cancer cell line.
MedLine Citation:
PMID:  8603367     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A human cervical cancer cell line, CX, was established from a patient with squamous cell carcinoma of the uterine cervix. The CX cells were epithelial in morphology with relatively large vesicular nuclei, and prominent nucleoli. Cytoplasmic organelles were generally sparse but tonofilaments were relatively abundant. The cells grew as a compact sheet with close membrane approximation interconnected by desmosome-like junctions. CX cells contained cytokeratin, but not vimentin. Elevated levels of squamous cell carcinoma antigen and carcinoembryonic antigen were detected in the cell supernatants. Population doubling time was estimated to be about 20 h. CX cells were not able to grow in soft agar and not tumorigenic in nude mice. Chromosome analysis revealed that CX cells were heterogeneous and mainly had a female diploid karyotype. Unlike cervical cancer cell lines published previously, CX cells were demonstrated to be human papillomavirus-negative, p53 mutation-negative. Based on the distinct characteristics, CX cell line may prove to be a useful tool for the study of human cervical carcinogenesis.
Authors:
C Y Chou; Y H Chen; C C Tzeng; Y C Cheng; C F Chang; T M Chen
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer letters     Volume:  102     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-05-16     Completed Date:  1996-05-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  173-81     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, National Cheng Kung University Medical College, Tainan, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Antigens, Neoplasm / blood
Base Sequence
Carcinoembryonic Antigen / blood
Cell Adhesion / physiology
Cell Division / physiology
Female
Genes, p53*
Histocytochemistry
Humans
Karyotyping
Molecular Sequence Data
Mutation*
Papillomaviridae*
Polymerase Chain Reaction
Serpins*
Tumor Cells, Cultured*
Uterine Cervical Neoplasms / genetics,  pathology*,  virology*
Chemical
Reg. No./Substance:
0/Antigens, Neoplasm; 0/Carcinoembryonic Antigen; 0/Serpins; 0/squamous cell carcinoma-related antigen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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